Rabbit CBP20 Polyclonal Antibody | anti-CBP20 antibody

CBP20 (R21) Polyclonal Antibody

Cat. Num. AAA3002041

• Gene Names: NCBP2; CBC2; NIP1; CBP20; PIG55
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CBP20; Polyclonal Antibody; CBP20 (R21) Polyclonal Antibody; Nuclear cap-binding protein subunit 2; 20 kDa nuclear cap-binding protein; Cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; NCBP-interacting protein 1; NIP1; NCBP2; ORF Names: PIG55; anti-CBP20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CBP20 (R21) polyclonal antibody detects endogenous levels of CBP20 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 103

• Applicable Applications for anti-CBP20 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human CBP20.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CBP20 (R21) pAb at 1:1000 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CBP20 (R21) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using CBP20 (R21) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using CBP20 (R21) # MBS3002041. “up-arrow” (supernatant) ; “down-arrow (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of CBP20 (R21) pAb at 1:500 dillutionLane1:Hcc827 whole cell lysateLane2:A549 whole cell lysateLane3:The Brain tissue lysate of MouseLane4:The Brain tissue lysate of RatLane5:Hela whole cell lysate)

Related Product Information for anti-CBP20 antibody

In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80.

NCBI and Uniprot Product Information

• NCBI GI #: 110349727
• NCBI GeneID: 22916
• NCBI Accession #: NP_001036005.1
• NCBI GenBank Nucleotide #: NP_001036005.1
• UniProt Accession #: P52298
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: nuclear cap-binding protein subunit 2 isoform 2
• NCBI Official Synonym Full Names: nuclear cap binding protein subunit 2
• NCBI Official Symbol: NCBP2
• NCBI Official Synonym Symbols: CBC2; NIP1; CBP20; PIG55
• NCBI Protein Information: nuclear cap-binding protein subunit 2
• UniProt Protein Name: Nuclear cap-binding protein subunit 2
• UniProt Gene Name: NCBP2
• UniProt Synonym Gene Names: CBP20; CBP20; NIP1
• UniProt Entry Name: NCBP2_HUMAN

NCBI Description

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NCBP2: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA- mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. Belongs to the RRM NCBP2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; mRNA cap complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding; RNA 7-methylguanosine cap binding; nucleotide binding; RNA cap binding

Biological Process: transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis; viral reproduction; positive regulation of viral transcription; positive regulation of RNA export from nucleus; RNA splicing; histone mRNA metabolic process; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; mRNA capping; RNA elongation from RNA polymerase II promoter; mRNA catabolic process, nonsense-mediated decay; snRNA export from nucleus; gene expression; RNA-mediated gene silencing; nuclear mRNA cis splicing, via U2-type spliceosome; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of translational initiation