Rabbit anti-Human MAVS Polyclonal Antibody | anti-MAVS antibody

Anti-MAVS Antibody, Rabbit Polyclonal

Cat. Num. AAA8108114

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MAVS; Polyclonal Antibody; Anti-MAVS Antibody; Rabbit Polyclonal; MAVS Antibody; Rabbit PAb; Antigen Affinity Purified; mitochondrial antiviral signaling protein; Anti-CARDIF Antibody; Anti-IPS-1 Antibody; Anti-IPS1 Antibody; Anti-VISA Antibody; anti-MAVS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MAVS
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MAVS antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:50-1:200
• Immunogen: E Coli-derived Human MAVS fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MAVS fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MAVS in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MAVS in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MAVS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MAVS polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-MAVS antibody

MAVS, as a signal molecule, performs an essential function in the development of antiviral immune response. The molecule of MAVS consists of two domains: the N-terminal domain and the C-terminal domain. The N-terminal end of MAVS contains the caspase activation and recruitment domain (CARD). CARD is responsible for MAVS interaction with RIG-I and MDA-5, which act as cytosolic sensors detecting foreign viral genetic material in the host cell. After binding to viral RNA, RIG-I or MDA-5 activates MAVS and transmits the signal of IFN type I gene expression. The C-terminal transmembrane domain (TM) of MAVS anchors the protein to the outer mitochondrial membrane. MAVS localizes in the membrane of mitochondria and peroxisomes and mitochondrial-associated endoplasmic reticulum membranes. Structural and functional studies have revealed that MAVS activity relies on the formation of functional high molecular weight prion-like aggregates.

References

Zamorano Cuervo N, et al. (2018) Virus infection triggers mavs polymers of distinct molecular weight. Viruses 10 (2): 56.Wyzewski Z, et al. (2016) [mavs protein and its interactions with hepatitis a, b and c viruses]. Postepy Hig Med Dosw (Online) 70 14-24.Dai T, et al. (2018) Faf1 regulates antiviral immunity by inhibiting mavs but is antagonized by phosphorylation upon viral infection. Cell Host Microbe 24 (6): 776-790.e775.