RYK sirna

RYK siRNA (Mouse)

Cat. Num. AAA8222328

• Gene Names: Ryk; Vik; ERK-3; AW536699
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RYK; RYK siRNA (Mouse); MRK; Tyrosine-protein kinase RYK; Kinase VIK; Met-related kinase; NYK-R; RYK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RYK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 591

• Applicable Applications for RYK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RYK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RYK sirna

siRNA to inhibit RYK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110681702
• NCBI GeneID: 20187
• NCBI Accession #: NP_001036072.1
• NCBI GenBank Nucleotide #: NM_001042607.1
• UniProt Accession #: Q01887; Q61890
• Molecular Weight: 66,285 Da
• NCBI Official Full Name: tyrosine-protein kinase RYK isoform 2
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: Ryk
• NCBI Official Synonym Symbols: Vik; ERK-3; AW536699
• NCBI Protein Information: tyrosine-protein kinase RYK
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Ryk
• UniProt Synonym Gene Names: Mrk
• UniProt Entry Name: RYK_MOUSE

Uniprot Description

RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Ryk family

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: transferase activity; Wnt-protein binding; protein binding; Wnt receptor activity; transmembrane receptor activity; frizzled binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: axon guidance; Wnt receptor signaling pathway; negative chemotaxis; signal transduction; negative regulation of axon extension involved in axon guidance; protein amino acid phosphorylation; corpus callosum development; neuron differentiation; axonogenesis; positive regulation of MAPKKK cascade; skeletal morphogenesis; phosphorylation; neurite development