Rabbit anti-Human, Mouse Ryk Polyclonal Antibody | anti-Ryk antibody

Ryk, ID (Ryk, Mrk, Tyrosine-protein kinase RYK, Kinase VIK, Met-related kinase, NYK-R) (APC)

Cat. Num. AAA6344602

• Gene Names: Ryk; Vik; ERK-3; AW536699
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ryk; Polyclonal Antibody; ID (Ryk; Mrk; Tyrosine-protein kinase RYK; Kinase VIK; Met-related kinase; NYK-R) (APC); NYK-R; anti-Ryk antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Ryk antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Ryk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-348 amino acids from the Central region of mouse Ryk.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Ryk Antibody (Center) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the Ryk antibody detected the Ryk protein (arrow).)

Related Product Information for anti-Ryk antibody

Ryk is potential growth factor receptor protein tyrosine kinase.

Product Categories/Family for anti-Ryk antibody

Antibodies; Protein Kinases

References

Buchert, M., et al. PLoS Genet. 6 (1), E1000816 (2010): Lyu, J., et al. Dev. Cell 15(5):773-780(2008). Tamplin, O.J., et al. BMC Genomics 9, 511 (2008): Keeble, T.R., et al. J. Neurosci. 26(21):5840-5848(2006). Schmitt, A.M., et al. Nature 439(7072):31-37(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 110681702
• NCBI GeneID: 20187
• NCBI Accession #: NP_001036072.1
• NCBI GenBank Nucleotide #: NM_001042607.1
• UniProt Accession #: Q01887; Q61890
• Molecular Weight: 70 kDa
• NCBI Official Full Name: tyrosine-protein kinase RYK isoform 2
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: Ryk
• NCBI Official Synonym Symbols: Vik; ERK-3; AW536699
• NCBI Protein Information: tyrosine-protein kinase RYK; NYK-R; kinase VIK; met-related kinase; growth factor receptor
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Ryk
• UniProt Synonym Gene Names: Mrk
• UniProt Entry Name: RYK_MOUSE

Uniprot Description

Function: May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with DVL1 (via PDZ domain). Ref.7

Subcellular location: Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm. Note: In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus. Ref.10

Tissue specificity: Is detected in all the tissues. Highest levels are seen in the ovary, lung and placenta.

Developmental stage: Highest expression at E16 when callosal axons are beginning to cross the midline. At E17-E18, when the majority of axons are projecting away from the midline, expression is observed but at a lower level. Present on the cell bodies of neurons in cortical layers at E18. Ref.9 Ref.10

Domain: The extracellular WIF domain is responsible for Wnt binding. Ref.7

Post-translational modification: Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 1 protein kinase domain.Contains 1 WIF domain.

Sequence caution: The sequence AAA40079.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA40079.1 differs from that shown. Reason: Frameshift at positions 17 and 28.