Western Blot (WB)
(Western blot analysis of PNGase expression in HeLa (A), mouse liver (B), rat testis (C) whole cell lysates.)
Rabbit PNGase Polyclonal Antibody | anti-PNGase antibody
Anti-PNGase Antibody
Western Blot (WB)
(Western blot analysis of PNGase expression in HeLa (A), mouse liver (B), rat testis (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PNGase expression in HeLa (A), mouse liver (B), rat testis (C) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
Uniprot Description
NGLY1: Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Belongs to the transglutaminase-like superfamily. PNGase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.1.52; Hydrolase
Chromosomal Location of Human Ortholog: 3p24.2
Cellular Component: cytoplasm
Molecular Function: protein binding; peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity; metal ion binding
Biological Process: glycoprotein catabolic process
Disease: Congenital Disorder Of Deglycosylation
Research Articles on PNGase
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Product Notes
The PNGase ngly1 (Catalog #AAA8307433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PNGase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PNGase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PNGase ngly1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNGase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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