S100A4 sirna

S100A4 siRNA (Mouse)

Cat. Num. AAA8217327

• Gene Names: S100a4; 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: S100A4; S100A4 siRNA (Mouse); CAPL; MTS1; Protein S100-A4; Metastasin; Metastatic cell protein; PEL98; Placental calcium-binding protein; Protein 18A2; Protein Mts1; S100 calcium-binding protein A4; S100A4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: S100A4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 101

• Applicable Applications for S100A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse S100A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for S100A4 sirna

siRNA to inhibit S100A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33859624
• NCBI GeneID: 20198
• NCBI Accession #: NP_035441.1
• NCBI GenBank Nucleotide #: NM_011311.2
• UniProt Accession #: P07091; P20066
• NCBI Official Full Name: protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein A4
• NCBI Official Symbol: S100a4
• NCBI Official Synonym Symbols: 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
• NCBI Protein Information: protein S100-A4
• UniProt Protein Name: Protein S100-A4
• Protein Family: Protein
• UniProt Gene Name: S100a4
• UniProt Synonym Gene Names: Capl; Mts1
• UniProt Entry Name: S10A4_MOUSE

Uniprot Description

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis

Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; RAGE receptor binding; protein binding; metal ion binding; calcium ion binding; actin binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade