S100A4 recombinant protein

S100A4 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122773

• Gene Names: S100a4; 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
• Purity: >95% as determined by SDS-PAGE
• Synonyms: S100A4; S100A4 Protein; Mouse; Recombinant (hFc Tag); Mouse S100A4 Protein (Fc Tag); 18A2 Protein; 42a Protein; Capl Protein; FSp1 Protein; metastasin Protein; Mts1 Protein; PeL98 Protein; pk9a Protein; S100 calcium binding protein A4; S100A4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Lys101

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse S100A4 (P07091)(Ala2-Lys101) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for S100A4 recombinant protein

Background: S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin beta, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.

Product Categories/Family for S100A4 recombinant protein

Cancer Drug Targets Calcium-binding Proteins and Related Molecules

References

Ambartsumian N. et al., 1995, Gene. 159: 125-30. Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22. Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8. Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30. Boye K. et al., 2008, Int J Cancer. 123: 1301-10. Garrett SC. et al., 2006, J Biol Chem. 281: 677-80. Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.

NCBI and Uniprot Product Information

• NCBI GI #: 33859624
• NCBI GeneID: 20198
• NCBI Accession #: NP_035441.1
• NCBI GenBank Nucleotide #: NM_011311.2
• UniProt Accession #: P07091; P20066
• Molecular Weight: 11,721 Da
• NCBI Official Full Name: protein S100-A4
• NCBI Official Synonym Full Names: S100 calcium binding protein A4
• NCBI Official Symbol: S100a4
• NCBI Official Synonym Symbols: 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
• NCBI Protein Information: protein S100-A4; S100 calcium-binding protein A4; calvasculin; metastatic cell protein; placental calcium-binding protein; protein 18A2; protein Mts1
• UniProt Protein Name: Protein S100-A4
• Protein Family: Protein
• UniProt Gene Name: S100a4
• UniProt Synonym Gene Names: Capl; Mts1
• UniProt Entry Name: S10A4_MOUSE

Uniprot Description

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis

Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; metal ion binding; calcium ion binding; actin binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade