Rabbit anti-Human, Mouse RASA4 Polyclonal Antibody | anti-RASA4 antibody

RASA4 Antibody

Cat. Num. AAA9610121

• Gene Names: RASA4; GAPL; CAPRI
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RASA4; Polyclonal Antibody; RASA4 Antibody; Ca2+ promoted Ras inactivator; Calcium promoted Ras inactivator; CAPRI; GAPL; GTPase activating protein like; KIAA0538; MGC131890; Ras GTPase activating protein 4; RAS p21 protein activator 4; RasGAP activating like protein 2; anti-RASA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RASA4 antibody detects endogenous levels of total RASA4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 757

• Applicable Applications for anti-RASA4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human RASA4
• Subcellular Location: Cytoplasm; Cytosol. Cell Membrane; Peripheral Membrane Protein. Note=Localized to the cytosol as a result of its lack of phosphoinositide binding activity. Upon agonist-stimulated calcium mobilization, utilizes the C2A and C2B domains to associate with the plasma membrane.
• Tissue Specificity: Widely expressed.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RASA4 using RAW264.7 whole cell lysates)

Related Product Information for anti-RASA4 antibody

Function: Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.
Similarity: The PH domain does not bind phosphatidylinositol 4, 5-bisphosphate or phosphatidylinositol 3, 4, 5-trisphosphate. This lack of binding activity is due to Leu-592, compared to Arg found in other family members.

NCBI and Uniprot Product Information

• NCBI GI #: 299829175
• NCBI GeneID: 10156
• NCBI Accession #: NP_001073346.2
• NCBI GenBank Nucleotide #: NM_001079877.2
• UniProt Accession #: O43374; O60286; Q14CQ4; Q86UW3; Q96QU0
• Molecular Weight: Observed: 90 kDa; Predicted: 91 kDa
• NCBI Official Full Name: ras GTPase-activating protein 4 isoform 2
• NCBI Official Synonym Full Names: RAS p21 protein activator 4
• NCBI Official Symbol: RASA4
• NCBI Official Synonym Symbols: GAPL; CAPRI
• NCBI Protein Information: ras GTPase-activating protein 4
• UniProt Protein Name: Ras GTPase-activating protein 4
• Protein Family: Ras GTPase-activating protein
• UniProt Gene Name: RASA4
• UniProt Synonym Gene Names: CAPRI; GAPL; KIAA0538

NCBI Description

This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.