ANGPTL3 sirna

ANGPTL3 siRNA (Mouse)

Cat. Num. AAA8216015

• Gene Names: Angptl3; hypl
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ANGPTL3; ANGPTL3 siRNA (Mouse); Angiopoietin-related protein 3; Angiopoietin-like protein 3; ANGPTL3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ANGPTL3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 455

• Applicable Applications for ANGPTL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ANGPTL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ANGPTL3 sirna

siRNA to inhibit ANGPTL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33469117
• NCBI GeneID: 30924
• NCBI Accession #: NP_038941.1
• NCBI GenBank Nucleotide #: NM_013913.4
• UniProt Accession #: Q9R182
• Molecular Weight: 52,543 Da
• NCBI Official Full Name: angiopoietin-related protein 3 preproprotein
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: Angptl3
• NCBI Official Synonym Symbols: hypl
• NCBI Protein Information: angiopoietin-related protein 3
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl3
• UniProt Entry Name: ANGL3_MOUSE

NCBI Description

This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Inhibitor; Secreted, signal peptide; Cell adhesion; Motility/polarity/chemotaxis; Secreted

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome; extracellular region

Molecular Function: enzyme inhibitor activity; integrin binding; phospholipase inhibitor activity; growth factor activity

Biological Process: cholesterol metabolic process; glycerol metabolic process; phospholipid catabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; signal transduction; fatty acid metabolic process; cholesterol homeostasis; sequestering of lipid; positive regulation of angiogenesis; triacylglycerol metabolic process; acylglycerol homeostasis; phospholipid metabolic process; negative regulation of catalytic activity; artery morphogenesis; positive regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; phospholipid homeostasis; positive regulation of cell migration