TRPC4AP sirna
TRPC4AP siRNA (Human)
Gene Names
TRPC4AP; TRUSS; TRRP4AP; PPP1R158; C20orf188
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
TRPC4AP; TRPC4AP siRNA (Human); C20orf188; TRRP4AP; Short transient receptor potential channel 4-associated protein; Trp4-associated protein; Trpc4-associated protein; Protein TAP1; TNF-receptor ubiquitous scaffolding/signaling protein; Protein TRUSS; TRPC4AP sirna
Host
Synthetic
Reactivity
Human
Specificity
TRPC4AP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
797
Applicable Applications for TRPC4AP sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPC4AP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for TRPC4AP sirna
siRNA to inhibit TRPC4AP expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,043 Da
NCBI Official Full Name
short transient receptor potential channel 4-associated protein isoform a
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily C, member 4 associated protein
NCBI Official Symbol
TRPC4AP
NCBI Official Synonym Symbols
TRUSS; TRRP4AP; PPP1R158; C20orf188
NCBI Protein Information
short transient receptor potential channel 4-associated protein
UniProt Protein Name
Short transient receptor potential channel 4-associated protein
UniProt Gene Name
TRPC4AP
UniProt Synonym Gene Names
C20orf188; TRRP4AP; Trp4-associated protein; Trpc4-associated protein; Protein TRUSS
UniProt Entry Name
TP4AP_HUMAN
Uniprot Description
TRPC4AP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: plasma membrane
Molecular Function: protein binding; phosphatase binding
Biological Process: ubiquitin-dependent protein catabolic process; protein ubiquitination; transmembrane transport
Research Articles on TRPC4AP
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Product Notes
The TRPC4AP trpc4ap (Catalog #AAA8202714) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The TRPC4AP siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRPC4AP can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the TRPC4AP trpc4ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPC4AP, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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