Western Blot (WB)
(The extracts of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human PNPO Monoclonal Antibody | anti-PNPO antibody
PNPO antibody
Gene Names
PNPO; PDXPO; HEL-S-302
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-G affinity chromatography
Synonyms
PNPO; Monoclonal Antibody; PNPO antibody; Pyridoxine-5'-phosphate oxidase; FLJ10535; PDXPO; anti-PNPO antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT2C7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
261
Applicable Applications for anti-PNPO antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500 ~ 1:5000.
Recommended starting dilution is 1:1000
Recommended starting dilution is 1:1000
Antigen Species
Human
Immunogen
Recombinant human PNPO (57-261aa) purified from E. coli
Clone Note
Anti-human PNPO mAb, clone AT2C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PNPO protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The extracts of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PNPO antibody
PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.
Product Categories/Family for anti-PNPO antibody
References
Ngo EO, et al. (Jun 1998). Biochemistry 37 (21): 7741-8.; Mills PB, Surtees RA, et al. (2005). Hum. Mol. Genet. 14 (8): 1077-86.; Kang JH, et al. (Jun 2004). Eur J Biochem 271 (12): 2452-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,200 Da
NCBI Official Full Name
pyridoxine-5'-phosphate oxidase
NCBI Official Synonym Full Names
pyridoxamine 5'-phosphate oxidase
NCBI Official Symbol
PNPO
NCBI Official Synonym Symbols
PDXPO; HEL-S-302
NCBI Protein Information
pyridoxine-5'-phosphate oxidase
UniProt Protein Name
Pyridoxine-5'-phosphate oxidase
Protein Family
UniProt Gene Name
PNPO
NCBI Description
The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]
Uniprot Description
Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).
Research Articles on PNPO
Similar Products
Product Notes
The PNPO pnpo (Catalog #AAA200200) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PNPO antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the PNPO pnpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNPO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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