Frizzled 2 Recombinant Protein | FZD2 recombinant protein

Frizzled 2 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122483

• Gene Names: Fzd2; Fz10; Fzd10; Mfz10; Mfz10a; AL033370; AW456835
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Frizzled 2; Frizzled 2 Protein; Mouse; Recombinant (hFc Tag); Mouse Frizzled-2/FZD2 Protein (Fc Tag); AL033370 Protein; AW456835 Protein; Fz10 Protein; Fzd10 Protein; Mfz10 Protein; Mfz10a Protein; frizzled class receptor 2; FZD2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln29-Leu168

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Fzd2 (NP_065256.1)(Gln29-Leu168) was expressed with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for FZD2 recombinant protein

Background: FZD2 may be a tumor suppressor gene in salivary adenoid cystic carcinomas (SACCs) that inhibits cell growth and migration. OMOD2 is a rare skeletal dysplasia and characterized by facial dysmorphism and shortness of the upper extremities and first metacarpal bones, and heterozygous FZD2 mutations may be disease-causing for OMOD2. heterozygous de novo mutation (G434V) in the frizzled class receptor 2 (FZD2) gene in a patient with distinct facial features including hypertelorism, bilateral cleft lip/palate, short nose with a broad nasal bridge, microretrognathia, and bilateral shortness of the upper limbs.

Product Categories/Family for FZD2 recombinant protein

Non-Canonical Wnt Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 10048406
• NCBI GeneID: 57265
• NCBI Accession #: NP_065256.1
• NCBI GenBank Nucleotide #: NM_020510.2
• UniProt Accession #: Q9JIP6; Q810M0; Q9JIP5; Q9WUJ2
• Molecular Weight: 64,059 Da
• NCBI Official Full Name: frizzled-2
• NCBI Official Synonym Full Names: frizzled homolog 2 (Drosophila)
• NCBI Official Symbol: Fzd2
• NCBI Official Synonym Symbols: Fz10; Fzd10; Mfz10; Mfz10a; AL033370; AW456835
• NCBI Protein Information: frizzled-2; fz-2; mFz2; fz-10; frizzled-10
• UniProt Protein Name: Frizzled-2
• UniProt Gene Name: Fzd2
• UniProt Synonym Gene Names: Fzd10; Fz-2; mFz2; Fz-10; mFz10
• UniProt Entry Name: FZD2_MOUSE

Uniprot Description

Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Subcellular location: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Expressed in embryonic and adult heart, lung, chondrocytes and brain. Also expressed in the developing gastrointestinal tract (strongest in foregut), much weaker expression in the adult. No expression in fetal liver and adult spleen. Up-regulated in esophageal squamous cell carcinomas.

Domain: Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway

By similarity.The FZ domain is involved in binding with Wnt ligands

By similarity.

Post-translational modification: Ubiquitinated by ZNRF3, leading to its degradation by the proteasome

By similarity.

Sequence similarities: Belongs to the G-protein coupled receptor Fz/Smo family.Contains 1 FZ (frizzled) domain.