RIZ1 Recombinant Protein | PRDM2 recombinant protein

RIZ1 Protein, Human, Recombinant

Cat. Num. AAA8120739

• Gene Names: PRDM2; RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
• Purity: >96% as determined by SDS-PAGE
• Synonyms: RIZ1; RIZ1 Protein; Human; Recombinant; Human PRDM2/RIZ1 Protein; HUMHOXY1 Protein; KMT8 Protein; MTB-ZF Protein; RIZ Protein; RIZ2 Protein; PR domain containing 2; with ZNF domain; PRDM2 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM DTT, 0.5mM GSH, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala200

• Species: Human
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2)(Met 1-Ala 200), containing the SET domian, was expressed, with two additonal amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PRDM2 recombinant protein

Background: PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is an S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR(SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and acts as a transcriptional activator. Besides, PRDM2 (RIZ) can bind to the retinoblastoma protein (RB) and also Interacts with GATA3.

References

Buyes, I.M. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 4467-4471. Muraosa, Y. et al., 1996, Eur. J. Biochem. 235: 471-479. Kim, K. et al., 2003, Cancer. Res. 63: 7619-7623. Shapiro, V.S. et al., 1995, Gene. 163: 329-330. Briknarova, K.et al.,2008,Biochem.Biophys.Res.Commun.366:807-813.

NCBI and Uniprot Product Information

• NCBI GI #: 20336258
• NCBI GeneID: 7799
• NCBI Accession #: NP_036363.2
• NCBI GenBank Nucleotide #: NM_012231.4
• UniProt Accession #: Q13029; Q13149; Q14550; Q5THJ1; Q5VUL9; B1AJZ4; B5MC68
• Molecular Weight: 162,107 Da
• NCBI Official Full Name: PR domain zinc finger protein 2 isoform a
• NCBI Official Synonym Full Names: PR/SET domain 2
• NCBI Official Symbol: PRDM2
• NCBI Official Synonym Symbols: RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
• NCBI Protein Information: PR domain zinc finger protein 2
• UniProt Protein Name: PR domain zinc finger protein 2
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM2
• UniProt Synonym Gene Names: KMT8; RIZ

NCBI Description

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PRDM2: S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: Golgi apparatus; nucleus

Molecular Function: DNA binding transcription factor activity; histone-lysine N-methyltransferase activity; zinc ion binding

Biological Process: determination of adult lifespan; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription from RNA polymerase II promoter