Western Blot (WB)
(Western blot analysis of HeLa (heat shocked; lane 2), Vero (lane 3), CHO-K1 (lane 4) and ESK-4 (lane 5) cell lysates probed with Mouse anti vesicle-fusing ATPase . Molecular weight marker is shown in lane 1)
Mouse Vesicle-fusing ATPase Monoclonal Antibody | anti-NSF antibody
Mouse anti Vesicle-fusing ATPase
Gene Names
NSF; SKD2; SEC18
Reactivity
Human, Rat, Bovine, Hamster, Monkey, Pig, Mouse, Rabbit, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography on Protein G
Synonyms
Vesicle-fusing ATPase; Monoclonal Antibody; Mouse anti Vesicle-fusing ATPase; Vesicle-fusing ATPase Antibody; anti-NSF antibody
Host
Mouse
Reactivity
Human, Rat, Bovine, Hamster, Monkey, Pig, Mouse, Rabbit, Sheep
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9G7-3
Purity/Purification
Purified by affinity chromatography on Protein G
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3), 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
744
Applicable Applications for anti-NSF antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
Recombinant bovine vesicle-fusing ATPase
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the murine NS-1 myeloma.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of HeLa (heat shocked; lane 2), Vero (lane 3), CHO-K1 (lane 4) and ESK-4 (lane 5) cell lysates probed with Mouse anti vesicle-fusing ATPase . Molecular weight marker is shown in lane 1)
Western Blot (WB)
(Western blot analysis of HeLa (heat shocked; lane 2), Vero (lane 3), CHO-K1 (lane 4) and ESK-4 (lane 5) cell lysates probed with Mouse anti vesicle-fusing ATPase . Molecular weight marker is shown in lane 1)
Related Product Information for anti-NSF antibody
Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 recognizes vesicle-fusing ATPase also known as NEM-sensitive fusion protein or NSF.
Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself.
Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
71,584 Da
NCBI Official Full Name
Vesicle-fusing ATPase
NCBI Official Synonym Full Names
N-ethylmaleimide sensitive factor, vesicle fusing ATPase
NCBI Official Symbol
NSF
NCBI Official Synonym Symbols
SKD2; SEC18
NCBI Protein Information
vesicle-fusing ATPase
UniProt Protein Name
Vesicle-fusing ATPase
Protein Family
UniProt Gene Name
NSF
UniProt Synonym Gene Names
NEM-sensitive fusion protein
Uniprot Description
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling ().
Research Articles on NSF
Similar Products
Product Notes
The NSF nsf (Catalog #AAA225377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Vesicle-fusing ATPase reacts with Human, Rat, Bovine, Hamster, Monkey, Pig, Mouse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Vesicle-fusing ATPase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the NSF nsf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vesicle-fusing ATPase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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