Rabbit anti-Human FASLG / Fas Ligand Polyclonal Antibody | anti-FASLG antibody

Rabbit Polyclonal to Human FASLG / Fas Ligand

Cat. Num. AAA244219

• Gene Names: FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: FASLG / Fas Ligand; Polyclonal Antibody; Rabbit Polyclonal to Human FASLG / Fas Ligand; Anti-FASLG / Fas Ligand Antibody IHC-plus; FASLG; ALPS1B; Apoptosis antigen ligand; APTL; CD95L; CD95 ligand; CD178 antigen; CD95-L; Fas antigen ligand; Fas ligand; FASL; TNFSF6; APT1LG1; CD178; Human FASLG; Fas Ligand; anti-FASLG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human Fas Ligand.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
• Concentration: 0.96 mg/ml (varies by lot)
• Sequence Length: 281

• Applicable Applications for anti-FASLG antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000)
• Target Species: Human
• Immunogen Description: Synthetic peptide containing a sequence corresponding to a region within amino acids 82 and 145 of Human Fas Ligand.
• Immunogen Type: Synthetic peptide
• Immunogen: FASLG / Fas Ligand antibody was raised against synthetic peptide containing a sequence corresponding to a region within amino acids 82 and 145 of Human Fas Ligand.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FASLG / FASL antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FASLG / FASL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. FASLG antibody diluted at 1:1000.)

Related Product Information for anti-FASLG antibody

Apoptosis antigen ligand 1 is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with its ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in TNFSF6 may be related to some cases of systemic lupus erythematosus (SLE).

Product Categories/Family for anti-FASLG antibody

Family: TNF

NCBI and Uniprot Product Information

• NCBI GI #: 4557329
• NCBI GeneID: 356
• NCBI Accession #: NP_000630.1
• NCBI GenBank Nucleotide #: NM_000639.2
• UniProt Accession #: P48023; Q9BZP9
• Molecular Weight: 14,006 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: FASLG
• NCBI Official Synonym Symbols: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: FASLG
• UniProt Synonym Gene Names: APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_HUMAN

NCBI Description

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: lysosomal lumen; extracellular space; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; extracellular region; caveola; nucleus; external side of plasma membrane

Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; cell-cell signaling; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response

Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome