Rabbit PRDM2 Polyclonal Antibody | anti-PRDM2 antibody

PRDM2 (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RI

Cat. Num. AAA6337063

• Gene Names: PRDM2; RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRDM2; Polyclonal Antibody; PRDM2 (PRDM2; KMT8; RIZ; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RI; Zinc finger protein RIZ; anti-PRDM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PRDM2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC: 1:100-500; ELISA: 1:1,000; IP: 1 ug Pab/100 ug cell lysate; Applications are based on unconjugated antibody.
• Immunogen: Purified recombinant GST fusion protein encoding a portion within aa 245-573 of rat Riz1. This antibody also recognizes the isoform beta (Riz2) of PRDM2 (185 kDa).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells. Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis.)

Related Product Information for anti-PRDM2 antibody

PRDM2 is a 250-kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. Using fluorescence in situ hybridization, the PRDM2 gene has been mapped to 1p36. PRDM2 may be a candidate target of 1p36 alterations that commonly occur in neuroendocrine, breast, liver, colon, skin, and lymphoid tumors.

Product Categories/Family for anti-PRDM2 antibody

Antibodies; Retinoblastoma Proteins

References

Muraosa, Y., et al., Eur. J. Biochem. 235(3):471-479 (1996). Buyse, I.M., et al., Proc. Natl. Acad. Sci. U.S.A. 92(10):4467-4471 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 55953110
• NCBI GeneID: 7799
• NCBI Accession #: NP_001007258.1
• NCBI GenBank Nucleotide #: NM_015866
• UniProt Accession #: Q13029; Q13149; Q14550; Q5THJ1; Q5VUL9; B1AJZ4; B5MC68
• Molecular Weight: 162,107 Da
• NCBI Official Full Name: PR domain zinc finger protein 2 isoform c
• NCBI Official Synonym Full Names: PR/SET domain 2
• NCBI Official Symbol: PRDM2
• NCBI Official Synonym Symbols: RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
• NCBI Protein Information: PR domain zinc finger protein 2
• UniProt Protein Name: PR domain zinc finger protein 2
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM2
• UniProt Synonym Gene Names: KMT8; RIZ

NCBI Description

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PRDM2: S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: Golgi apparatus; nucleus

Molecular Function: DNA binding transcription factor activity; histone-lysine N-methyltransferase activity; zinc ion binding

Biological Process: determination of adult lifespan; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription from RNA polymerase II promoter