HER3/ERBB3 ELISA Pair Set | ERBB3 elisa pair set
HER3/ERBB3 Matched ELISA Antibody Pair Set, Mouse
Synonyms
HER3/ERBB3; HER3/ERBB3 Matched ELISA Antibody Pair Set; Mouse; Mouse ERBB3/HER3 ELISA Pair Set; erb-b2 receptor tyrosine kinase 3; C76256 Matched ELISA Antibody Pair Set; Erbb-3 Matched ELISA Antibody Pair Set; Erbb-3r Matched ELISA Antibody Pair Set; Erbb3 Matched ELISA Antibody Pair Set; Erbb3r Matched ELISA Antibody Pair Set; Her3 Matched ELISA Antibody Pair Set; ERBB3 elisa pair set
Specificity
Quantitative determination of Mouse HER3/ERBB3
Assay Type
Solid Phase Sandwich ELISA
Detection Range
39.06-2500pg/mL
Sensitivity
39.06pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse ERBB3/HER3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse ERBB3/HER3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 57 ng of recombinant Mouse ERBB3/HER3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse ERBB3/HER3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 57 ng of recombinant Mouse ERBB3/HER3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Related Product Information for ERBB3 elisa pair set
ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
This HER3/ERBB3 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse HER3/ERBB3. It contains Mouse HER3/ERBB3 capture antibody, Mouse HER3/ERBB3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse HER3/ERBB3 protein.
This HER3/ERBB3 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse HER3/ERBB3. It contains Mouse HER3/ERBB3 capture antibody, Mouse HER3/ERBB3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse HER3/ERBB3 protein.
Product Categories/Family for ERBB3 elisa pair set
References
Kraus M.H., et al., (1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
Katoh M., et al., (1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
Katoh M., et al., (1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
