Testing Data
Her2/ERBB2 ELISA Pair Set | ERBB2 elisa pair set
Her2/ERBB2 Matched ELISA Antibody Pair Set, Human
Synonyms
Her2/ERBB2; Her2/ERBB2 Matched ELISA Antibody Pair Set; Human; Human HER2/ErbB2/CD340 ELISA Pair Set; erb-b2 receptor tyrosine kinase 2; CD340 Matched ELISA Antibody Pair Set; ENV Matched ELISA Antibody Pair Set; ENVW Matched ELISA Antibody Pair Set; ERVWE1 Matched ELISA Antibody Pair Set; HER-2 Matched ELISA Antibody Pair Set; HER-2/neu Matched ELISA Antibody Pair Set; HER2 Matched ELISA Antibody Pair Set; HERV-7q Matched ELISA Antibody Pair Set; HERV-W-ENV Matched ELISA Antibody Pair Set; HERV7Q Matched ELISA Antibody Pair Set; HERVW Matched ELISA Antibody Pair Set; HERVWENV Matched ELISA Antibody Pair Set; MLN 19 Matched ELISA Antibody Pair Set; MLN19 Matched ELISA Antibody Pair Set; NEU Matched ELISA Antibody Pair Set; NGL Matched ELISA Antibody Pair Set; TKR1 Matched ELISA Antibody Pair Set; ERBB2 elisa pair set
Specificity
Quantitative determination of Human Her2/ERBB2
Assay Type
Solid Phase Sandwich ELISA
Detection Range
24-1500pg/mL
Sensitivity
24pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human HER2/ErbB2/CD340 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1ug/mL in PBS before coating.
2. Detection Antibody 0.1 mg/mL of rabbit anti-Human HER2/ErbB2/CD340 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 110 ng of recombinant Human HER2/ErbB2/CD340. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
2. Detection Antibody 0.1 mg/mL of rabbit anti-Human HER2/ErbB2/CD340 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 110 ng of recombinant Human HER2/ErbB2/CD340. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for ERBB2 elisa pair set
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
This Her2/ERBB2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Her2/ERBB2. It contains Human Her2/ERBB2 capture antibody, Human Her2/ERBB2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Her2/ERBB2 protein.
This Her2/ERBB2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Her2/ERBB2. It contains Human Her2/ERBB2 capture antibody, Human Her2/ERBB2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Her2/ERBB2 protein.
Product Categories/Family for ERBB2 elisa pair set
References
Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
Similar Products
Product Notes
The ERBB2 (Catalog #AAA8118549) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Her2/ERBB2, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
