Rabbit anti-Human CCL2 Polyclonal Antibody

CCL2, CT (C-C motif Chemokine 2, Small-inducible Cytokine A2, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic Protein 1, MCP-1, Monocyte Chemotactic And Activating Factor, MCAF, Monocyte Secretory Protein JE, HC11, SCYA2, MCP1)

Cat. Num. AAA643931

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CCL2; Polyclonal Antibody; CT (C-C motif Chemokine 2; Small-inducible Cytokine A2; Monocyte Chemoattractant Protein 1; Monocyte Chemotactic Protein 1; MCP-1; Monocyte Chemotactic And Activating Factor; MCAF; Monocyte Secretory Protein JE; HC11; SCYA2; MCP1); Anti -CCL2; anti-CCL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCL2.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CCL2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100; Immunofluorescence: 1:10-1:50; Flow cytometry: 1:10-1:50
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the C-terminal region of human CCL2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CCL2 Antibody (C-term) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of CCL2 Antibody (C-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CCL2 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCL2 antibody

Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Product Categories/Family for anti-CCL2 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 112361886
• NCBI Official Full Name: CCL2
• Protein Family: C-C motif chemokine