Testing Data
LILRA3 elisa pair set
LILRA3 Matched ELISA Antibody Pair Set, Human
Synonyms
LILRA3; LILRA3 Matched ELISA Antibody Pair Set; Human; Human ILT6/LILRA3 ELISA Pair Set; leukocyte immunoglobulin like receptor A3; CD85e Matched ELISA Antibody Pair Set; HM31 Matched ELISA Antibody Pair Set; HM43 Matched ELISA Antibody Pair Set; ILT-6 Matched ELISA Antibody Pair Set; ILT6 Matched ELISA Antibody Pair Set; LIR-4 Matched ELISA Antibody Pair Set; LIR4 Matched ELISA Antibody Pair Set; LILRA3 elisa pair set
Specificity
Quantitative determination of Human LILRA3
Assay Type
Solid Phase Sandwich ELISA
Detection Range
156.25-10000pg/mL
Sensitivity
156.25pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human ILT6/LILRA3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human ILT6/LILRA3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 436 ng of recombinant Human ILT6/LILRA3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human ILT6/LILRA3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 436 ng of recombinant Human ILT6/LILRA3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for LILRA3 elisa pair set
ILT6, also known as LILRA3, belongs to the ILT family. In humans, the ILT gene family includes up to 11 members. The extracellular portion of all members includes at least two and usually four immunoglobulin domains. ILT-2 through 5 are all inhibitory members having variable numbers of cytoplasmic ITIM domains. ILT6 lacks a transmembrane domain. The function of ILT6 is currently unknown. however it is highly homologous to other LILR genes, and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the ILT6 might impair interactions of membrane-bound LILRs (such as LILRB1, an inhibitory receptor expressed on effector and memory CD8 T cells) with their HLA ligands, thus modulating immune reactions and influencing susceptibility to disease.
This LILRA3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LILRA3. It contains Human LILRA3 capture antibody, Human LILRA3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LILRA3 protein.
This LILRA3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LILRA3. It contains Human LILRA3 capture antibody, Human LILRA3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LILRA3 protein.
Product Categories/Family for LILRA3 elisa pair set
References
Wi?niewski A, et al. (2004) Distribution of LILRA3 (ILT6/LILRA3/LIR4) deletion in psoriatic patients and healthy controls. Hum Immunol. 64(4):458-61. Norman PJ, et al. (2003) DNA sequence variation and molecular genotyping of natural killer leukocyte immunoglobulin-like receptor, LILRA3. Immunogenetics. 55(3):165-71. Cella M, et al. (1997) A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing. J Exp Med. 185(10): 1743-51.
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Product Notes
The LILRA3 (Catalog #AAA8118452) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LILRA3, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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