Rabbit anti-Human EPS15 Polyclonal Antibody | anti-EPS15 antibody

EPS15, CT (Epidermal Growth Factor Receptor Substrate 15, Protein Eps15, AF-1p protein, AF1P)

Cat. Num. AAA629129

• Gene Names: EPS15; AF1P; AF-1P; MLLT5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: EPS15; Polyclonal Antibody; CT (Epidermal Growth Factor Receptor Substrate 15; Protein Eps15; AF-1p protein; AF1P); Anti -EPS15; anti-EPS15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EPS15.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-EPS15 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human EPS15 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EPS15 Pab in HL60 cell line lysate (35ug/lane). EPS15 (arrow) was detected using the purified Pab.)

Related Product Information for anti-EPS15 antibody

EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.

Product Categories/Family for anti-EPS15 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4503593
• NCBI GeneID: 2060
• NCBI Accession #: NP_001972.1
• NCBI GenBank Nucleotide #: NM_001981.2
• UniProt Accession #: P42566; Q5SRH4; B2R8J7; D3DPJ2
• Molecular Weight: 98,656 Da
• NCBI Official Full Name: epidermal growth factor receptor substrate 15 isoform A
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 15
• NCBI Official Symbol: EPS15
• NCBI Official Synonym Symbols: AF1P; AF-1P; MLLT5
• NCBI Protein Information: epidermal growth factor receptor substrate 15; protein AF-1p; ALL1 fused gene from chromosome 1
• UniProt Protein Name: Epidermal growth factor receptor substrate 15
• Protein Family: Epidermal growth factor receptor substrate
• UniProt Gene Name: EPS15
• UniProt Synonym Gene Names: AF1P; Protein Eps15
• UniProt Entry Name: EPS15_HUMAN

NCBI Description

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]

Uniprot Description

EPS15: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits. Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM1 or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: membrane; intracellular membrane-bound organelle; early endosome membrane; cytoplasm; plasma membrane; AP-2 adaptor complex; coated pit; cytosol

Molecular Function: identical protein binding; protein binding; calcium ion binding; polyubiquitin binding; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; cell proliferation; clathrin cage assembly; protein transport; vesicle organization and biogenesis; Golgi to endosome transport; endocytosis; endocytic recycling