SerpinB9 cell lysate
Human SerpinB9 Baculovirus-Insect cells Overexpression Lysate
Gene Names
SERPINB9; PI9; CAP3; PI-9; CAP-3
Applications
Western Blot
Synonyms
SerpinB9; Human SerpinB9 Baculovirus-Insect cells Overexpression Lysate; Human SerpinB9/CAP-3 Insect Cell Lysate (WB positive control); serpin peptidase inhibitor; clade B (ovalbumin); member 9; Human CAP-3 Overexpression Lysate; Human CAP3 Overexpression Lysate; Human PI-9 Overexpression Lysate; Human PI9 Overexpression Lysate; Human Serpin B9 Overexpression Lysate; SerpinB9 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
394aa
Sequence
Glu2-Pro376
Applicable Applications for SerpinB9 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SERPINB9 (P50453) (Glu 2-Pro 376) was expressed, with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SerpinB9 cell lysate
SerpinB9, also known as Cytoplasmic antiproteinase 3, CAP-3, Peptidase inhibitor 9, SERPINB9 and PI-9, is a cytoplasm protein that belongs to the serpin family and Ov-serpin subfamily. Serpin-B9 (CAP-3/PI-9) is the only known human intracellular inhibitor of granzyme B (GrB), the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Serpin-B9 and SPI-6 expression in immune-privileged cells, APCs, and CTLs protect these cells against the actions of granzyme B, and when expressed in tumor cells or virally infected hepatocytes, confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 (CAP-3/PI-9) in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin/granzyme pathway and then through the Fas/FasL pathway. Serpin-B9 (CAP-3/PI-9) is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B. Upregulated expression of Serpin-B9 (CAP-3/PI-9) in NSCLC cells may serve to protect them from apoptosis induced by GrB.
This Human SerpinB9 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB9 protein from the overexpression lysate was verified.
This Human SerpinB9 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SerpinB9 protein from the overexpression lysate was verified.
References
Rowshani, A.T. et al., 2005, J Infect Dis.192 (11): 1908-11. Cunningham, T.D. et al., 2007, Cell Immunol 245 (1): 32-41. Rousalova, I. et al., 2010, Int J Oncol 36 (1): 275-83. Stout-Delgado, H.W. et al., 2007, J Immunol. 179 (10):6561-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,404 Da
NCBI Official Full Name
serpin B9
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 9
NCBI Official Symbol
SERPINB9
NCBI Official Synonym Symbols
PI9; CAP3; PI-9; CAP-3
NCBI Protein Information
serpin B9; peptidase inhibitor 9; cytoplasmic antiproteinase 3; protease inhibitor 9 (ovalbumin type); serpin peptidase inhibitor, clade B, member 9; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9
UniProt Protein Name
Serpin B9
Protein Family
UniProt Gene Name
SERPINB9
UniProt Synonym Gene Names
PI9; CAP-3; CAP3; PI-9
UniProt Entry Name
SPB9_HUMAN
NCBI Description
This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]
Uniprot Description
SERPINB9: Granzyme B inhibitor. Belongs to the serpin family. Ov-serpin subfamily.
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: extracellular space; membrane; cytoplasm; intracellular; nucleus; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; caspase inhibitor activity
Biological Process: mast cell mediated immunity; response to bacterium; immune response; negative regulation of caspase activity; negative regulation by symbiont of host apoptosis; protection from natural killer cell mediated cytotoxicity; negative regulation of apoptosis
Research Articles on SerpinB9
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Product Notes
The SerpinB9 serpinb9 (Catalog #AAA8114994) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 394aa. AAA Biotech's SerpinB9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SerpinB9 serpinb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu2-Pro37 6. It is sometimes possible for the material contained within the vial of "SerpinB9, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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