Mouse anti-Human Proteinase Inhibitor 9 (PI-9) Monoclonal Antibody | anti-SERPINB9 antibody

Proteinase Inhibitor 9 (PI-9) Antibody (Clone PI9-17)

Cat. Num. AAA840015

• Gene Names: SERPINB9; PI9; CAP3; PI-9; CAP-3
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Proteinase Inhibitor 9 (PI-9); Monoclonal Antibody; Proteinase Inhibitor 9 (PI-9) Antibody (Clone PI9-17); CAP3 CAP3 Cytoplasmic antiase3 PI9 Protease inhibitor9 ovalbumin type Serpin B9 Serine or Cysteine ase Inhibitor; anti-SERPINB9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: PI9-17
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) mouse monoclonal antibody from culture supernatant diluted in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 376

• Applicable Applications for anti-SERPINB9 antibody: Western Blot (WB)
• Application Notes: Western blotting (2-4 ug/ml) and Immunohistochemistry (20 ug/ml). It recognizes 42 kDa native human PI-9 and the 63 kDa PI-9 complexed to granzyme B. Reactivity to other species has not been tested.
• Immunogen: Recombinant human full length PI9
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-SERPINB9 antibody

Background: Proteinase inhibitor 9 (PI-9, also designated cytoplasmic antiproteinase 3, or CAP3) is a 42 kDa member of the ovalbumin family of serpins that is expressed in placenta, lung and cytotoxic lymphocytes. PI-9 is a potent inhibitor of granzyme B and of granzyme B-mediated apoptosis, and is also an inhibitor of capase-1 and, to a smaller extent, caspase-4 and caspase-8. PI-9 expression is also upregulated in response to inflammatory stimuli. This upregulation protects cells from apoptosis induced by endogenously expressed or released granzyme B, particulary during target cell killling.

NCBI and Uniprot Product Information

• NCBI GI #: 4758906
• NCBI GeneID: 5272
• NCBI Accession #: NP_004146.1
• NCBI GenBank Nucleotide #: NM_004155.5
• UniProt Accession #: P50453; Q5TD03; B2RBW3
• Molecular Weight: 42,404 Da
• NCBI Official Full Name: serpin B9
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• NCBI Official Symbol: SERPINB9
• NCBI Official Synonym Symbols: PI9; CAP3; PI-9; CAP-3
• NCBI Protein Information: serpin B9
• UniProt Protein Name: Serpin B9
• Protein Family: Serpin
• UniProt Gene Name: SERPINB9
• UniProt Synonym Gene Names: PI9; CAP-3; CAP3; PI-9
• UniProt Entry Name: SPB9_HUMAN

NCBI Description

This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

SERPINB9: Granzyme B inhibitor. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: extracellular space; membrane; cytoplasm; intracellular; nucleus; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; caspase inhibitor activity

Biological Process: mast cell mediated immunity; response to bacterium; immune response; negative regulation of caspase activity; negative regulation by symbiont of host apoptosis; protection from natural killer cell mediated cytotoxicity; negative regulation of apoptosis