Rabbit anti-Human PRCP Polyclonal Antibody | anti-PRCP antibody

PRCP (PCP, Lysosomal Pro-X Carboxypeptidase, Angiotensinase C, Lysosomal Carboxypeptidase C, Proline Carboxypeptidase, Prolylcarboxypeptidase, MGC2202) (AP)

Cat. Num. AAA6390538

• Gene Names: PRCP; PCP; HUMPCP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRCP; Polyclonal Antibody; PRCP (PCP; Lysosomal Pro-X Carboxypeptidase; Angiotensinase C; Lysosomal Carboxypeptidase C; Proline Carboxypeptidase; Prolylcarboxypeptidase; MGC2202) (AP); anti-PRCP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRCP.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PRCP antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PRCP, aa1-496 (NP_005031.1).
• Immunogen Sequence: MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRCP expression in transfected 293T cell line by PRCP polyclonal antibody. Lane 1: PRCP transfected lysate (55.8kD). Lane 2: Non-transfected lysate)

Related Product Information for anti-PRCP antibody

The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Product Categories/Family for anti-PRCP antibody

Antibodies; Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 4826940
• NCBI GeneID: 5547
• NCBI Accession #: NP_005031.1
• NCBI GenBank Nucleotide #: NM_005040
• UniProt Accession #: P42785; A8MU24; B2R7B7; B3KRK5; B5BU34
• Molecular Weight: 55,800 Da
• NCBI Official Full Name: lysosomal Pro-X carboxypeptidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: prolylcarboxypeptidase (angiotensinase C)
• NCBI Official Symbol: PRCP
• NCBI Official Synonym Symbols: PCP; HUMPCP
• NCBI Protein Information: lysosomal Pro-X carboxypeptidase; angiotensinase C; proline carboxypeptidase; lysosomal carboxypeptidase C; prolylcarboxypeptidase isoform 1 preproprotein
• UniProt Protein Name: Lysosomal Pro-X carboxypeptidase
• Protein Family: Lysosomal Pro-X carboxypeptidase
• UniProt Gene Name: PRCP
• UniProt Synonym Gene Names: PCP; PRCP
• UniProt Entry Name: PCP_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.

Protein type: EC 3.4.16.2; Protease

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: lysosome; plasma membrane

Molecular Function: serine carboxypeptidase activity; protein binding

Biological Process: plasma kallikrein-kinin cascade; negative regulation of systemic arterial blood pressure; regulation of blood vessel endothelial cell migration; glucose homeostasis; proteolysis; blood coagulation; angiogenesis involved in wound healing; blood coagulation, intrinsic pathway