EDA2R cell lysate
Human EDA2R HEK293 Overexpression Lysate
Gene Names
EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
Applications
Western Blot
Synonyms
EDA2R; Human EDA2R HEK293 Overexpression Lysate; Human XEDAR/EDA2R HEK293 Cell Lysate (WB positive control); ectodysplasin A2 receptor; Human EDA-A2R Overexpression Lysate; Human EDAA2R Overexpression Lysate; Human TNFRSF27 Overexpression Lysate; Human XEDAR Overexpression Lysate; EDA2R cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
149aa
Sequence
Met1-Thr138
Applicable Applications for EDA2R cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human EDA2R (NP_068555.1) extracellular domain (Met 1-Thr 138) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for EDA2R cell lysate
Tumor necrosis factor receptor superfamily member 27, also known as X-linked ectodysplasin-A2 receptor, EDA-A2 receptor, EDA2R, XEDAR and TNFRSF27, is a single-pass type III membrane protein. TNFRSF27/EDA2R contains threeTNFR-Cys repeats. It is a new member of the tumor necrosis factor receptor family that has been shown to be highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2). TNFRSF27/EDA2R is a receptor for EDA isoform A2, but not for EDA isoform A1. TNFRSF27/EDA2R mediates the activation of the NF-kappa-B and JNK pathways. The activation seems to be mediated by binding to TRAF3 and TRAF6. Ectodysplasin, a member of the tumor necrosis factor family, is encoded by the anhidrotic ectodermal dysplasia EDA gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that differ only by an insertion of two amino acids. This insertion functions to determine receptor binding specificity, such that EDA-A1 binds only the receptor EDAR, whereas EDA-A2 binds only the related, but distinct, X-linked ectodysplasin-A2 receptor (XEDAR).
This Human EDA2R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EDA2R protein from the overexpression lysate was verified.
This Human EDA2R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of EDA2R protein from the overexpression lysate was verified.
References
Yan M., et al., 2000, Science. 290: 523-527. Sinha S.K., et al., 2002, J. Biol. Chem. 277: 44953-44961. Prodi, D.A. et al., 2008, J Invest Dermatol 128 (9):2268-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,959 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 27 isoform 1
NCBI Official Synonym Full Names
ectodysplasin A2 receptor
NCBI Official Symbol
EDA2R
NCBI Official Synonym Symbols
XEDAR; EDAA2R; EDA-A2R; TNFRSF27
NCBI Protein Information
tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 27
Protein Family
UniProt Gene Name
EDA2R
UniProt Synonym Gene Names
TNFRSF27; XEDAR; EDA-A2 receptor
UniProt Entry Name
TNR27_HUMAN
NCBI Description
EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]
Uniprot Description
EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: Xq12
Cellular Component: integral to plasma membrane; integral to membrane
Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity
Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor
Research Articles on EDA2R
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Product Notes
The EDA2R eda2r (Catalog #AAA8114802) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 149aa. AAA Biotech's EDA2R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the EDA2R eda2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr13 8. It is sometimes possible for the material contained within the vial of "EDA2R, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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