Rabbit anti-Human UBE2G1 Polyclonal Antibody | anti-UBE2G1 antibody

UBE2G1, CT (UBE2G1, UBE2G, Ubiquitin-conjugating enzyme E2 G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1) (PE)

Cat. Num. AAA6360683

• Gene Names: UBE2G1; UBC7; E217K; UBE2G
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2G1; Polyclonal Antibody; CT (UBE2G1; UBE2G; Ubiquitin-conjugating enzyme E2 G1; E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1) (PE); Ubiquitin-protein ligase G1; anti-UBE2G1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-UBE2G1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the C-terminal region of human UBE2G1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UBE2G1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the UBE2G1 antibody detected the UBE2G1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of UBE2G1 (arrow) using rabbit polyclonal UBE2G1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2G1 gene.)

Product Categories/Family for anti-UBE2G1 antibody

Antibodies; Ubiquitin Ligases

References

Hassink, G., et al. Biochem. J. 388 (PT 2), 647-655 (2005): Kim, B.W., et al. Mol. Endocrinol. 17(12):2603-2612(2003). Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003). Imai, Y., et al. Cell 105(7):891-902(2001). Tiwari, S., et al. J. Biol. Chem. 276(19):16193-16200(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13489085
• NCBI GeneID: 7326
• NCBI Accession #: NP_003333.1
• NCBI GenBank Nucleotide #: NM_003342.4
• UniProt Accession #: P62253; Q99462; B2R7P2; D3DTK0
• Molecular Weight: 19,509 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 G1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 G1
• NCBI Official Symbol: UBE2G1
• NCBI Official Synonym Symbols: UBC7; E217K; UBE2G
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 G1
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 G1
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2G1
• UniProt Synonym Gene Names: UBE2G
• UniProt Entry Name: UB2G1_HUMAN

Uniprot Description

UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cellular protein catabolic process; ubiquitin-dependent protein catabolic process