LAIR2 cell lysate
Human LAIR2 HEK293 Overexpression Lysate
Gene Names
LAIR2; CD306
Applications
Western Blot
Synonyms
LAIR2; Human LAIR2 HEK293 Overexpression Lysate; Human LAIR2/CD306 HEK293 Cell Lysate (WB positive control); leukocyte associated immunoglobulin like receptor 2; Human CD306 Overexpression Lysate; Human LAIR2 Overexpression Lysate; Human MGC71634 Overexpression Lysate; Human XXbac-BCX535A19.6 Overexpression Lysate; LAIR2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
131aa
Sequence
Met1-Pro152
Applicable Applications for LAIR2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human LAIR2 (NP_002279.2) (Met 1-Pro 152) was expressed and purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LAIR2 cell lysate
Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2), also known as CD36, is a 131 amino acid protein containing one Ig-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. Accordingly, LAIR2 is suggested to perform an immunoregulatory function.
This Human LAIR2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LAIR2 protein from the overexpression lysate was verified.
This Human LAIR2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LAIR2 protein from the overexpression lysate was verified.
Product Categories/Family for LAIR2 cell lysate
References
Meyaard, L. et al., 1997, Immunity.7:283-290. Meyaard, L. et al., 1999, J. Immunol. 162:5800-5804. Meyaard, L. et al., 2001, J. Exp. Med. 194 (1): 107-112. Meyaard, L., 2003, J Biol Regul Homeost Agents. 17 (4): 330-333. Lebbink, R. J. et al., 2008, J Immunol. 180 (3):1662-1669. Lebbink, R.J. et al., 2009, Matrix Biol. 28 (4):202-210.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,722 Da
NCBI Official Full Name
leukocyte-associated immunoglobulin-like receptor 2 isoform a
NCBI Official Synonym Full Names
leukocyte-associated immunoglobulin-like receptor 2
NCBI Official Symbol
LAIR2
NCBI Official Synonym Symbols
CD306
NCBI Protein Information
leukocyte-associated immunoglobulin-like receptor 2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated Ig-like receptor-2
UniProt Protein Name
Leukocyte-associated immunoglobulin-like receptor 2
Protein Family
UniProt Gene Name
LAIR2
UniProt Synonym Gene Names
CD306; LAIR-2
UniProt Entry Name
LAIR2_HUMAN
NCBI Description
The protein encoded by this gene is a member of the immunoglobulin superfamily. It was identified by its similarity to leukocyte-associated immunoglobulin-like receptor 1, a membrane-bound receptor that modulates innate immune response. The protein encoded by this locus is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. This gene maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
LAIR-2: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: extracellular region
Research Articles on LAIR2
Similar Products
Product Notes
The LAIR2 lair2 (Catalog #AAA8114190) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 131aa. AAA Biotech's LAIR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LAIR2 lair2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro15 2. It is sometimes possible for the material contained within the vial of "LAIR2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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