HGF Activator/HGFA Cell Lysate | HGFAC cell lysate
Human HGF Activator/HGFA HEK293 Overexpression Lysate
Gene Names
HGFAC; HGFA
Applications
Western Blot
Synonyms
HGF Activator/HGFA; Human HGF Activator/HGFA HEK293 Overexpression Lysate; Human HGFA HEK293 Cell Lysate (WB positive control); Human HGFA Overexpression Lysate; Human MGC138395 Overexpression Lysate; Human MGC138397 Overexpression Lysate; Human RP11-529E10.2 Overexpression Lysate; HGFAC cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
631aa
Sequence
Met1-Ser655
Applicable Applications for HGFAC cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human HGF activator precursor (NP_001519.1) (Met 1-Ser 655) was expressed with a fused polyhistidine-tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for HGFAC cell lysate
HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. Besides, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in the liver, kidney, and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.
This Human HGF Activator/HGFA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HGF Activator/HGFA protein from the overexpression lysate was verified.
This Human HGF Activator/HGFA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HGF Activator/HGFA protein from the overexpression lysate was verified.
Product Categories/Family for HGFAC cell lysate
References
1. Shimomura, T. et al., 1993, J. Biol. Chem. 268: 22927-22932. 2. Miyazawa, K. et al., 1996, J. Biol. Chem. 271 : 3615-3618. 3. Shia, S. et al., 2005, J. Mol. Biol. 346: 1335-1349. 4. Kataoka, H. et al., 2000, J. Biol. Chem. 275: 40453-40462. 5. Tjin, E.P. et al., 2006, Blood. 107: 760-768. 6. Kitajima, Y. et al., 2000, Cancer. Res. 60: 6148-6159.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,682 Da
NCBI Official Full Name
hepatocyte growth factor activator isoform 2 preproprotein
NCBI Official Synonym Full Names
HGF activator
NCBI Official Symbol
HGFAC
NCBI Official Synonym Symbols
HGFA
NCBI Protein Information
hepatocyte growth factor activator
Protein Family
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Product Notes
The HGFAC (Catalog #AAA8113715) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 631aa. AAA Biotech's HGF Activator/HGFA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the HGFAC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser65 5. It is sometimes possible for the material contained within the vial of "HGF Activator/HGFA, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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