Rat Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit | HGFAC elisa kit

Rat Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit

Cat. Num. AAA2025080

• Gene Names: HGFAC; HGFA
• Reactivity: Rat
• Synonyms: Hepatocyte Growth Factor Activator (HGFAC); Rat Hepatocyte Growth Factor Activator (HGFAC) ELISA Kit; HGFA; HGFAP; HGF Activator; HGFAC elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor Activator (HGFAC).; No significant cross-reactivity or interference between Hepatocyte Growth Factor Activator (HGFAC) and analogues was observed.
• Sequence Length: 655

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor Activator (HGFAC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor Activator (HGFAC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGFAC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Activator (HGFAC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Activator (HGFAC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor Activator (HGFAC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HGFAC elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 4504383
• NCBI GeneID: 3083
• NCBI Accession #: NP_001519.1
• NCBI GenBank Nucleotide #: NM_001528.3
• Molecular Weight: 70,682 Da
• NCBI Official Full Name: hepatocyte growth factor activator isoform 2 preproprotein
• NCBI Official Synonym Full Names: HGF activator
• NCBI Official Symbol: HGFAC
• NCBI Official Synonym Symbols: HGFA
• NCBI Protein Information: hepatocyte growth factor activator
• UniProt Protein Name: Hepatocyte growth factor activator
• Protein Family: Hepatocyte growth factor activator
• UniProt Gene Name: HGFAC
• UniProt Synonym Gene Names: HGF activator; HGFA
• UniProt Entry Name: HGFA_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis