Typical Testing Data/Standard Curve (for reference only)
Human HGF activator ELISA Kit | HGFAC elisa kit
Human hepatocyte growth factor activator, HGFA ELISA Kit
Gene Names
HGFAC; HGFA
Reactivity
Human
Synonyms
HGF activator; Human hepatocyte growth factor activator; HGFA ELISA Kit; Human hepatocyte growth factor activator (HGFA) ELISA kit; RP11-529E10.2; HGFA; MGC138395; MGC138397; ; HGFAC elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HFE. No significant cross-reactivity or interference between human HFE and analogues was observed.
Sequence Length
655
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates
Assay Type
Quantitative Sandwich
Detection Range
78 pg/ml-5000 pg/ml
Sensitivity
19.5 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HGFAC elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HFE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HFE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HFE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HFE bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,682 Da
NCBI Official Full Name
hepatocyte growth factor activator isoform 2 preproprotein
NCBI Official Synonym Full Names
HGF activator
NCBI Official Symbol
HGFAC
NCBI Official Synonym Symbols
HGFA
NCBI Protein Information
hepatocyte growth factor activator
UniProt Protein Name
Hepatocyte growth factor activator
Protein Family
UniProt Gene Name
HGFAC
UniProt Synonym Gene Names
HGF activator; HGFA
UniProt Entry Name
HGFA_HUMAN
NCBI Description
This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
Function: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Research Articles on HGFAC
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Product Notes
The Human HGFAC hgfac (Catalog #AAA701647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701647 ELISA Kit recognizes Human HGFAC. It is sometimes possible for the material contained within the vial of "HGF activator, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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