Western Blot (WB)
(Western Blot (WB) analysis of ANP32A polyclonal antibody)
Rabbit ANP32A Polyclonal Antibody | anti-ANP32A antibody
ANP32A Polyclonal Antibody
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ANP32A; Polyclonal Antibody; ANP32A Polyclonal Antibody; acidic (leucine-rich) nuclear phosphoprotein 32 family; member A; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; AN32A_HUMAN; C15orf1; Cerebellar leucine rich acidic nuclear protein; Hepatopoietin Cn; HPPCn; I1PP2A; Inhibitor 1 of protein phosphatase 2A; inhibitor-1 of protein phosphatase-2A; Lanp; Leucine Rich Acidic Nuclear Protein; Leucine-rich acidic nuclear protein; MAPM; Mapmodulin; MGC119787; MGC150373; PHAPI; Potent heat stable protein phosphatase 2A inhibitor I1PP2A; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; Putative HLA DR associated protein I; Putative HLA-DR-associated protein I; Putative Human HLA Class II Associated Protein; Putative human HLA class II associated protein I; Putative human HLA class II-associated protein; anti-ANP32A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ANP32A polyclonal antibody detects endogenous levels of ANP32A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
249
Applicable Applications for anti-ANP32A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human ANP32A.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ANP32A polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of ANP32A polyclonal antibody)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using ANP32A polyclonal antibody)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using ANP32A polyclonal antibody)
Related Product Information for anti-ANP32A antibody
Protein phosphatase 2A PP2A is a major mammalian protein serine/threonine phosphatase that regulates diverse cellular processes. ANP32A, also known as Inhibitor 1 of PP2A (I1PP2A), and inhibitor 2 of PP2A (I2PP2A), which share large sequence similarity, are heat-stable protein inhibitors of the cellular phosphatase activity of PP2A. ANP32A and I2PP2A were initially characterized as putative HLA class II associated proteins Phap I and Phap II. These inhibitor proteins act noncompetitively to selectively inhibit PP2A, but do not affect the phosphatase activity of the related proteins PP1, PP2B and PP2C. The ANP32A protein is localized to both the cytoplasm and the nucleus. In contrast, I2PP2A is located predominantly in the nucleus and is highly expressed in Wilms tumor cells. Transient expression of I2PP2A in HEK-293 cells leads to an increase in the DNA binding activity of the proto-oncogene c-Jun.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 28kDa
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member A
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member A
NCBI Official Symbol
ANP32A
NCBI Official Synonym Symbols
LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Protein Family
UniProt Gene Name
ANP32A
UniProt Synonym Gene Names
C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
UniProt Entry Name
AN32A_HUMAN
Uniprot Description
ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: nucleoplasm; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; gene expression; nucleocytoplasmic transport
Research Articles on ANP32A
Similar Products
Product Notes
The ANP32A anp32a (Catalog #AAA3011331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANP32A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ANP32A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ANP32A anp32a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANP32A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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