Western Blot (WB)
(Anti-LDHA rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:36 kDa)
Rabbit anti-Rat LDHA Polyclonal Antibody | anti-LDHA antibody
Anti-LDHA Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 0.5-2uL/mg of lysate
Western Blot (WB)
(Anti-LDHA rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:36 kDa)
Western Blot (WB)
(Anti-LDHA rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:36 kDa)
Immunoprecipitation (IP)
(rat LDHA was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate4 uL anti-rat LDHA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat LDHA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(rat LDHA was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate4 uL anti-rat LDHA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat LDHA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size: 35 kDa)
NCBI and Uniprot Product Information
NCBI Description
mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]
Uniprot Description
LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cytosol; membrane; mitochondrion; nucleus
Molecular Function: identical protein binding; kinase binding; L-lactate dehydrogenase activity; lactate dehydrogenase activity; NAD binding; protein binding
Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate metabolic process; NAD metabolic process; positive regulation of apoptosis; post-embryonic organ development; response to cAMP; response to drug; response to estrogen stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to nutrient; response to organic cyclic substance; substantia nigra development
Research Articles on LDHA
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Product Notes
The LDHA ldha (Catalog #AAA8113067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LDHA Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LDHA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the LDHA ldha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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