LDHA active protein

LDHA Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123490

• Gene Names: Ldha; CDK1; Ldh1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LDHA; LDHA Protein; Rat; Recombinant (His Tag); Rat LDH-A/LDHA Protein (His Tag); lactate dehydrogenase A; LDHA active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 50mM NaCl. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe332

• Species: Rat
• Activity: Measured by its ability to reduce pyruvate to lactate. The specific activity is >50,000pmoles/min/ug.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the rat Ldha (NP_058721.1)(Met1-Phe332) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LDHA active protein

Background: LDHA (Lactate Dehydrogenase A) is a Protein Coding gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA, a key enzyme regulating aerobic glycolysis, belongs to the lactate dehydrogenase family and is overexpressed in many human cancers, and correlates with poor clinical outcomes. LDHA can promote the Warburg effect to produce lactate and Adenosine Triphosphate (ATP) in aerobic conditions, which contributes to oncogenesis metastasis and drug resistance in various cancers. Up-regulation of lactate dehydrogenase LDHA is a frequent event in human malignancies and relate to poor postoperative outcome. Diseases associated with LDHA include Fanconi-Bickel Syndrome and Myoglobinuria.

NCBI and Uniprot Product Information

• NCBI GI #: 8393706
• NCBI GeneID: 24533
• NCBI Accession #: NP_058721.1
• NCBI GenBank Nucleotide #: NM_017025.1
• UniProt Accession #: P04642
• Molecular Weight: 36,451 Da
• NCBI Official Full Name: L-lactate dehydrogenase A chain
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: Ldha
• NCBI Official Synonym Symbols: CDK1; Ldh1
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldha
• UniProt Synonym Gene Names: Ldh-1; Ldh1; LDH-A; LDH-M

NCBI Description

mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytosol; membrane; mitochondrion; nucleus

Molecular Function: identical protein binding; kinase binding; L-lactate dehydrogenase activity; lactate dehydrogenase activity; NAD binding; protein binding

Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate metabolic process; NAD metabolic process; positive regulation of apoptosis; post-embryonic organ development; response to cAMP; response to drug; response to estrogen stimulus; response to glucose stimulus; response to hydrogen peroxide; response to hypoxia; response to nutrient; response to organic cyclic substance; substantia nigra development