LDHA active protein

Human Recombinant LDHA

Cat. Num. AAA845262

• Gene Names: LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: LDHA; Human Recombinant LDHA; L-lactate dehydrogenase A chain; GSD11; LDH1; LDHM; PIG19.; LDHA active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl and 20% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
• Sequence Length: 274

• Applicable Applications for LDHA active protein: SDS-PAGE
• Biological Activity: Specific activity is >20 units/mg.
• Results: >20 units/mg
• Unit Definition: One unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for LDHA active protein

Background: LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

Product Categories/Family for LDHA active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 207028494
• NCBI GeneID: 3939
• NCBI Accession #: NP_001128711.1
• NCBI GenBank Nucleotide #: NM_001135239.1
• UniProt Accession #: P00338; Q53G53; Q6IBM7; Q6ZNV1; Q9UDE8; Q9UDE9; B4DKQ2; B7Z5E3; D3DQY3; F8W819
• Molecular Weight: 38.8 kDa (352 aa, 1-332 aa + His-Tag)
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: LDHA
• NCBI Official Synonym Symbols: LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHA
• UniProt Synonym Gene Names: LDH-A; LDH-M
• UniProt Entry Name: LDHA_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - propanoate; Apoptosis; EC 1.1.1.27; Amino Acid Metabolism - cysteine and methionine; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: mitochondrion; membrane; nucleus; cytosol

Molecular Function: protein binding; L-lactate dehydrogenase activity

Biological Process: cellular metabolic process; glycolysis; substantia nigra development; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process

Disease: Glycogen Storage Disease Xi