Rabbit anti-Rat Ephrin-B1 Polyclonal Antibody | anti-EFNB1 antibody

Anti-Ephrin-B1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112760

• Gene Names: Efnb1; LERK2
• Reactivity: Rat
• Applications: ELISA
• Purity: Protein A
• Synonyms: Ephrin-B1; Polyclonal Antibody; Anti-Ephrin-B1 Antibody; Rabbit Polyclonal; Ephrin-B1/EFNB1 Antibody; Rabbit PAb; ephrin-B1; anti-EFNB1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat Ephrin-B1/EFNB1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EFNB1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Rat Ephrin-B1/EFNB1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat Ephrin-B1/EFNB1. Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-EFNB1 antibody

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

References

Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83.Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30.Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.

NCBI and Uniprot Product Information

• NCBI GI #: 1706670
• NCBI GeneID: 25186
• UniProt Accession #: P52796
• Molecular Weight: 37,951 Da
• NCBI Official Full Name: Ephrin-B1
• NCBI Official Synonym Full Names: ephrin B1
• NCBI Official Symbol: Efnb1
• NCBI Official Synonym Symbols: LERK2
• NCBI Protein Information: ephrin-B1; ELK-L; LERK-2; ELK ligand; EPH-related receptor tyrosine kinase ligand 2
• UniProt Protein Name: Ephrin-B1
• Protein Family: Ephrin
• UniProt Gene Name: Efnb1
• UniProt Synonym Gene Names: Eplg2; Lerk2; ELK-L; LERK-2
• UniProt Entry Name: EFNB1_RAT

NCBI Description

induces neurite outgrowth; may play a role in positive regulation of cerebellar development [RGD, Feb 2006]

Uniprot Description

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons ().