Ephrin-B1 Active Protein | EFNB1 active protein

Ephrin-B1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122840

• Gene Names: Efnb1; Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Ephrin-B1; Ephrin-B1 Protein; Mouse; Recombinant (His Tag); Mouse Ephrin-B1/EFNB1 Protein (His Tag); Cek5-L Protein; EFL-3 Protein; Elk-L Protein; Epl2 Protein; Eplg2 Protein; LERK-2 Protein; Lerk2 Protein; Stra1 Protein; ephrin-B1; EFNB1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser229

• Species: Mouse
• Activity: Measured by its ability to compete with mouse EphrinB1/Fc for binding to immobilized mouse EphB3 in a functional ELISA assay.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EFNB1 (NP_034240.1) extracellular domain (Met 1-Ser 229) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EFNB1 active protein

Background: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

References

Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev. 18(5): 572-83.Compagni A, et al. (2003) Control of skeletal patterning by ephrinB1-EphB interactions. Dev Cell. 5(2): 217-30.Wieland I, et al. (2004) Mutations of the ephrin-B1 gene cause craniofrontonasal syndrome. Am J Hum Genet. 74(6): 1209-15.

NCBI and Uniprot Product Information

• NCBI GI #: 6753726
• NCBI GeneID: 13641
• NCBI Accession #: NP_034240.1
• NCBI GenBank Nucleotide #: NM_010110.4
• UniProt Accession #: P52795; Q544L9
• Molecular Weight: 37,859 Da
• NCBI Official Full Name: ephrin-B1
• NCBI Official Synonym Full Names: ephrin B1
• NCBI Official Symbol: Efnb1
• NCBI Official Synonym Symbols: Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2
• NCBI Protein Information: ephrin-B1; Cek ligand; ELK ligand; Cek5 ligand; OTTMUSP00000019590; CEK5 receptor ligand; stimulated by retinoic acid gene 1 protein; EPH-related receptor tyrosine kinase ligand 2
• UniProt Protein Name: Ephrin-B1
• Protein Family: Ephrin
• UniProt Gene Name: Efnb1
• UniProt Synonym Gene Names: Epl2; Eplg2; Lerk2; Stra1
• UniProt Entry Name: EFNB1_MOUSE