Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) Standard Curve.)
Human Epidermal Growth Factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit | EGFL7 elisa kit
Human Epidermal Growth Factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
Gene Names
EGFL7; NEU1; ZNEU1; VE-STATIN
Reactivity
Human
Synonyms
Epidermal Growth Factor Like Domain Protein; Multiple 7 (EGFL7); Human Epidermal Growth Factor Like Domain Protein; Multiple 7 (EGFL7) ELISA Kit; EGFL7; MGC111117; RP11-251M1.2; VE-STATIN; ZNEU1; NEU1 protein; OTTHUMP00000022597; EGFL7 elisa kit
Reactivity
Human
Specificity
Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit has high sensitivity and excellent specificity for detection of Human EGFL7. No significant cross-reactivity or interference between Human EGFL7 and analogues was observed.
Sequence Length
273
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) Standard Curve.)
Related Product Information for EGFL7 elisa kit
Description: This Human Epidermal growth factor Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFL7 in samples. An antibody specific for EGFL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 275-amino acid protein has a calculated molecular mass of 29.8 kD. The mouse and human EGFL7 proteins both contain an N-terminal cleavable signal peptide followed by 2 epidermal growth factor (EGF)-like domains, and they share 78% amino acid identity. Northern blot analysis detected a transcript of about 1.6 kb only in mouse heart, lung, and kidney. In situ hybridization showed mouse Egfl7 expressed at embryonic day 7.5 exclusively in the primitive blood islands where the first endothelial cells differentiate, and later in endothelial cells of a wide range of tissues. Fluorescence-labeled Egfl7 was expressed in the endoplasmic reticulum of transfected mouse fibroblasts, and it was secreted into the culture medium.
Background: The deduced 275-amino acid protein has a calculated molecular mass of 29.8 kD. The mouse and human EGFL7 proteins both contain an N-terminal cleavable signal peptide followed by 2 epidermal growth factor (EGF)-like domains, and they share 78% amino acid identity. Northern blot analysis detected a transcript of about 1.6 kb only in mouse heart, lung, and kidney. In situ hybridization showed mouse Egfl7 expressed at embryonic day 7.5 exclusively in the primitive blood islands where the first endothelial cells differentiate, and later in endothelial cells of a wide range of tissues. Fluorescence-labeled Egfl7 was expressed in the endoplasmic reticulum of transfected mouse fibroblasts, and it was secreted into the culture medium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
epidermal growth factor-like protein 7
NCBI Official Synonym Full Names
EGF like domain multiple 7
NCBI Official Symbol
EGFL7
NCBI Official Synonym Symbols
NEU1; ZNEU1; VE-STATIN
NCBI Protein Information
epidermal growth factor-like protein 7
UniProt Protein Name
Epidermal growth factor-like protein 7
Protein Family
UniProt Gene Name
EGFL7
UniProt Synonym Gene Names
MEGF7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
NCBI Description
This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Uniprot Description
Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
Research Articles on EGFL7
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Product Notes
The Human EGFL7 egfl7 (Catalog #AAA9715607) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715607 ELISA Kit recognizes Human EGFL7. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Like Domain Protein, Multiple 7 (EGFL7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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