Ephrin-B1 / EFNB1 recombinant protein

Recombinant Human Ephrin-B1 / EFNB1 Protein (His & Fc tag)

Cat. Num. AAA2546026

• Gene Names: EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
• Purity: > (79.7+18.0) % as determined by SDS-PAGE
• Synonyms: Ephrin-B1 / EFNB1; Recombinant Human Ephrin-B1 / EFNB1 Protein (His & Fc tag); EFNB1; RP3-496G1.1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2; MGC8782; Ephrin-B1 / EFNB1 recombinant protein

• Host: Human Cells
• Purity/Purification: > (79.7+18.0) % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 346

• Application Notes: The recombinant human EFNB1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 64 and 36 KDa due to glycosylation.
• Predicted N Terminal: Leu 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for Ephrin-B1 / EFNB1 recombinant protein

Background: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Description: A DNA sequence encoding the human EFNB1 (NP_004420.1) extracellular domain (Met 1-Gly 232) was was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4758248
• NCBI GeneID: 1947
• NCBI Accession #: NP_004420.1
• NCBI GenBank Nucleotide #: NM_004429.4
• UniProt Accession #: P98172; D3DVU0
• Molecular Weight: 38,007 Da
• NCBI Official Full Name: ephrin-B1
• NCBI Official Synonym Full Names: ephrin B1
• NCBI Official Symbol: EFNB1
• NCBI Official Synonym Symbols: CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
• NCBI Protein Information: ephrin-B1
• UniProt Protein Name: Ephrin-B1
• UniProt Gene Name: EFNB1
• UniProt Synonym Gene Names: EFL3; EPLG2; LERK2; ELK-L; LERK-2

NCBI Description

The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNB1: a type I membrane protein of the ephrin family. A ligand of Eph-related receptor tyrosine kinases EphB1 and EphA1. Ephrins and ephrin receptors mediate numerous developmental processes, particularly in the nervous system. Ephrin-B1 may play a role in cell adhesion and functions in the development or maintenance of the nervous system. Binding to its receptor induces the collapse of commissural axons/growth cones in vitro. Induced by TNF-alpha. Expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: integral to plasma membrane; plasma membrane; synapse

Molecular Function: protein binding

Biological Process: axon guidance; cell adhesion; cell-cell signaling; ephrin receptor signaling pathway

Disease: Craniofrontonasal Syndrome