Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD59a expression on BABL/c bone marrow erythroid cells. Cells were stained with FITC-conjugated anti-Mouse CD59a. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of erythroid cells.)
Rabbit anti-Mouse CD59a Monoclonal Antibody | anti-CD59a antibody
Recombinant Anti-CD59a Antibody (FITC), Rabbit Monoclonal
Gene Names
Cd72; CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD59a; Monoclonal Antibody; Recombinant Anti-CD59a Antibody (FITC); Rabbit Monoclonal; CD59a/Protectin Antibody (FITC); Rabbit MAb; CD59a antigen; Anti-AA987121 Antibody; Anti-Cd59 Antibody; Anti-protectin Antibody; Anti-RP24-297H17.1 Antibody; anti-CD59a antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
133
Specificity
Mouse CD59a/Protectin
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD59a antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD59a/Protectin Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD59a/Protectin (rM CD59a/Protectin) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD59a expression on BABL/c bone marrow erythroid cells. Cells were stained with FITC-conjugated anti-Mouse CD59a. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of erythroid cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD59a expression on BABL/c bone marrow erythroid cells. Cells were stained with FITC-conjugated anti-Mouse CD59a. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of erythroid cells.)
Related Product Information for anti-CD59a antibody
Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins. It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.
References
Huang Y, et al. (2006) Defining the CD59-C9 binding interaction. J Biol Chem. 281 (37): 27398-404.Sawada R, et al. (1990) Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes. DNA Cell Biol. 9(3): 213-20.Philbrick WM, et al. (1990) The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur J Immunol. 20(1): 87-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,347 Da
NCBI Official Full Name
B-cell differentiation antigen CD72 isoform 2
NCBI Official Synonym Full Names
CD72 antigen
NCBI Official Symbol
Cd72
NCBI Official Synonym Symbols
CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
NCBI Protein Information
B-cell differentiation antigen CD72; lymphocyte antigen 32
UniProt Protein Name
B-cell differentiation antigen CD72
Protein Family
UniProt Gene Name
Cd72
UniProt Synonym Gene Names
Ly-32; Ly32; Lyb-2; Ly-32
UniProt Entry Name
CD72_MOUSE
Uniprot Description
Function: Plays a role in B-cell proliferation and differentiation.
Subunit structure: Homodimer; disulfide-linked. Associates with CD5. Interacts (tyrosine phosphorylated) with PTPN6/SHP-1. Ref.4
Subcellular location: Membrane; Single-pass type II membrane protein.
Tissue specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Post-translational modification: Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1. Ref.4
Sequence similarities: Contains 1 C-type lectin domain.
Research Articles on CD59a
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Product Notes
The CD59a cd72 (Catalog #AAA8111998) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD59a Antibody (FITC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD59a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD59a cd72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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