Mouse Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit | VEGFC elisa kit

Mouse Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit

Cat. Num. AAA2023969

• Gene Names: VEGFC; VRP; Flt4-L; LMPH1D
• Reactivity: Mouse
• Synonyms: Vascular Endothelial Growth Factor C (VEGFC); Mouse Vascular Endothelial Growth Factor C (VEGFC) ELISA Kit; VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein; VEGFC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGFC. No significant cross-reactivity or interference between VEGFC and analogues was observed.
• Sequence Length: 419

• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: <6.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFC in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGFC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFC, biotin-conjugated antibody and enzyme- onjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VEGFC elisa kit

Cytokine; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4885653
• NCBI GeneID: 7424
• NCBI Accession #: NP_005420.1
• NCBI GenBank Nucleotide #: NM_005429.4
• UniProt Accession #: P49767; B2R9Q8
• Molecular Weight: 46,883 Da
• NCBI Official Full Name: vascular endothelial growth factor C preproprotein
• NCBI Official Synonym Full Names: vascular endothelial growth factor C
• NCBI Official Symbol: VEGFC
• NCBI Official Synonym Symbols: VRP; Flt4-L; LMPH1D
• NCBI Protein Information: vascular endothelial growth factor C
• UniProt Protein Name: Vascular endothelial growth factor C
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFC
• UniProt Synonym Gene Names: VEGF-C; Flt4-L; VRP
• UniProt Entry Name: VEGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The encoded protein promotes angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. The proprotein is further cleaved into a fully processed form that can bind and activate VEGFR-2 and VEGFR-3 receptors. [provided by RefSeq, Apr 2014]

Uniprot Description

VEGFC: Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. Homodimer; non-covalent and antiparallel. Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; growth factor activity; vascular endothelial growth factor receptor 3 binding; chemoattractant activity

Biological Process: signal transduction; induction of positive chemotaxis; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; platelet degranulation; positive chemotaxis; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of cell proliferation; negative regulation of blood pressure; angiogenesis; positive regulation of cell-matrix adhesion; substrate-bound cell migration; response to drug; platelet activation; positive regulation of neuroblast proliferation; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of protein secretion; positive regulation of cell division; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation

Disease: Lymphedema, Hereditary, Id