Rabbit anti-Mouse Fas/CD95 Polyclonal Antibody | anti-FAS antibody

Anti-Fas/CD95 Antibody, Rabbit Polyclonal

Cat. Num. AAA8110808

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: Fas/CD95; Polyclonal Antibody; Anti-Fas/CD95 Antibody; Rabbit Polyclonal; FAS/CD95/APO-1/TNFRSF6 Antibody; Rabbit PAb; Fas cell surface death receptor; Anti-AI196731 Antibody; Anti-APO1 Antibody; Anti-APT1 Antibody; Anti-CD95 Antibody; Anti-lpr Antibody; Anti-TNFR6 Antibody; Anti-Tnfrsf6 Antibody; anti-FAS antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse FAS/CD95
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-FAS antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Mouse CD95 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CD95 (rM CD95). Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-FAS antibody

CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

Product Categories/Family for anti-FAS antibody

Tumor Suppressors; Death Receptor Signaling

References

Mschen M, et al. (2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80.Peter ME, et al. (2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35.Peter ME, et al. (2005) Does CD95 have tumor promoting activities Biochim Biophys Acta. 1755(1): 25-36.Chen L, et al. (2010) Cell death in the colonic epithelium during inflammatory bowel diseases: CD95/Fas and beyond. Inflamm Bowel Dis. 16(6): 1071-6.

NCBI and Uniprot Product Information

• NCBI GI #: 226443049
• NCBI GeneID: 14102
• NCBI Accession #: NP_032013.2
• NCBI GenBank Nucleotide #: NM_007987.2
• UniProt Accession #: P25446; Q6GT31; Q9DCQ1
• Molecular Weight: 37,437 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_MOUSE

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine; Cell surface; Apoptosis

Cellular Component: extracellular space; neuron projection; cell surface; extracellular region; integral to membrane; CD95 death-inducing signaling complex; lipid raft; secretory granule; membrane; cell soma; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; nucleus; sarcolemma; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; tumor necrosis factor receptor activity; transmembrane receptor activity; protease binding; protein complex binding; kinase binding

Biological Process: renal system process; apoptosis; positive regulation of apoptosis; response to toxin; regulation of lymphocyte differentiation; response to glucocorticoid stimulus; positive regulation of caspase activity; signal transduction; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; T cell homeostasis; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; spleen development; regulation of myeloid cell differentiation; transformed cell apoptosis; positive regulation of protein homooligomerization; negative regulation of B cell activation; negative thymic T cell selection; regulation of cell proliferation; dendrite regeneration; inflammatory cell apoptosis; B cell mediated immunity; neuron apoptosis; induction of apoptosis via death domain receptors; regulation of gene expression; immune response; gene expression; negative regulation of apoptosis