Rabbit anti-Human, Mouse RAN Polyclonal Antibody | anti-RAN antibody

RAN, ID (RAN, ARA24, GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein) (APC)

Cat. Num. AAA6340186

• Gene Names: RAN; TC4; Gsp1; ARA24
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAN; Polyclonal Antibody; ID (RAN; ARA24; GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein) (APC); Ras-related nuclear protein; anti-RAN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RAN antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human RAN.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAN Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the RAN antibody detected the RAN protein (arrow).)

Immunohistochemistry (IHC)

(RAN antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAN antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RAN Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RAN antibody

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Product Categories/Family for anti-RAN antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Kim, J.S., et al. Mol. Carcinog. 49(10):913-921(2010). Liu, C., et al. Mol. Cell. Biol. 30(16):3956-3969(2010). Boni, V., et al. Pharmacogenomics J. (2010). In press: Ly, T.K., et al. J. Biol. Chem. 285(8):5815-5826(2010). Mishra, R.K., et al. Nat. Cell Biol. 12(2):164-169(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5453555
• NCBI GeneID: 5901
• NCBI Accession #: NP_006316.1
• NCBI GenBank Nucleotide #: NM_006325.3
• UniProt Accession #: P62826; P17080; P28746; P28747; Q6IPB2; Q86V08; Q8NI90; Q9CSP3; Q9CWI7; Q9CZA2; Q9UDJ5; A8K3Z8; AAB24940
• Molecular Weight: 24,423 Da
• NCBI Official Full Name: GTP-binding nuclear protein Ran
• NCBI Official Synonym Full Names: RAN, member RAS oncogene family
• NCBI Official Symbol: RAN
• NCBI Official Synonym Symbols: TC4; Gsp1; ARA24
• NCBI Protein Information: GTP-binding nuclear protein Ran; RanGTPase; GTPase Ran; OK/SW-cl.81; ras-like protein TC4; member RAS oncogene family; ras-related nuclear protein; guanosine triphosphatase Ran; androgen receptor-associated protein 24
• UniProt Protein Name: GTP-binding nuclear protein Ran
• Protein Family: Ranakinin
• UniProt Gene Name: RAN
• UniProt Synonym Gene Names: ARA24
• UniProt Entry Name: RAN_HUMAN

NCBI Description

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

RAN: a small GTPase belonging to the RAS superfamily that regulates the nucleo-cytoplasmic traffic through the nuclear pore complex, the condensation of chromatin, spindle assembly and cytokinesis, as well as the organization of non-centrosomal microtubules. A major regulator of mitosis and a potential therapeutic target in cancers expressing higher Ras/MEK/ERK and PI3K/Akt/mTORC1 activities. Required for the import of protein into and RNA export from the nucleus. Cycles between the nucleus and cytoplasm in this process. The Ran guanine nucleotide-exchange factor (RanGEF) is nuclear, promoting the GTP-bound state, while the Ran GTPase activating protein (RanGAP) is cytoplasmic, promoting GDP-bound state. RanGDP is imported into the nucleus by nuclear transport factor-2 (NUTF2), where RanGEF catalyses nucleotide exchange and generates RanGTP. RanGTP then binds to transport factors, such as KPNB1 and CAS, and is exported to the cytoplasm, where RanGAP stimulates GTP hydrolysis. Regulates microtubule polymerization during mitosis; micritubule localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1), a Ran-GEF. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Regulates the formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. GTP-Ran interacts with survivin at S and M phases of the cell cycle. Ran-survivin complexes play a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Interacts with TERT following the tyrosine phosphorylation of TERT induced by H2O2, inducing nuclear export of TERT. Interacts with MAD2L2. Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis.

Protein type: Nuclear receptor co-regulator; G protein, monomeric, Ran; G protein; G protein, monomeric; Nuclear export; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: nucleoplasm; recycling endosome; membrane; cytoplasm; melanosome; nuclear pore; nucleus; cytosol; chromatin

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; androgen receptor binding; GTP binding; transcription coactivator activity; chromatin binding

Biological Process: ribosomal small subunit export from nucleus; mitosis; positive regulation of protein binding; viral reproduction; positive regulation of transcription, DNA-dependent; viral infectious cycle; signal transduction; ribosomal large subunit export from nucleus; mitotic spindle organization and biogenesis; cell division; protein import into nucleus; small GTPase mediated signal transduction; androgen receptor signaling pathway; gene expression; protein export from nucleus; actin cytoskeleton organization and biogenesis; DNA metabolic process; spermatid development