FAS / CD95 / APO-1 / TNFRSF6 recombinant protein

Recombinant Mouse FAS / CD95 / APO-1 / TNFRSF6 Protein (His tag)

Cat. Num. AAA2547202

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: FAS / CD95 / APO-1 / TNFRSF6; Recombinant Mouse FAS / CD95 / APO-1 / TNFRSF6 Protein (His tag); Fas; AI196731; APO1; APT1; CD95; TNFR6; Tnfrsf6; lpr; FAS / CD95 / APO-1 / TNFRSF6 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 327

• Application Notes: The recombinant mouse FAS consists of 159 amino acids and has a predicted molecular mass of 18 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFAS is approximately 30-35 kDa due to glycosylation.
• Predicted N Terminal: Gln 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FAS / CD95 / APO-1 / TNFRSF6 recombinant protein

Background: CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

Description: A DNA sequence encoding the extracellular domain of mouse FAS (NP_032013.2) (Met 1-Arg 169) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 226443049
• NCBI GeneID: 14102
• NCBI Accession #: NP_032013.2
• NCBI GenBank Nucleotide #: NM_007987.2
• Molecular Weight: 37,437 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell surface; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19 C1|19 29.48 cM

Cellular Component: apical plasma membrane; CD95 death-inducing signaling complex; cell soma; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; sarcolemma; secretory granule

Molecular Function: kinase binding; protease binding; protein binding; protein complex binding; tumor necrosis factor receptor activity

Biological Process: activated T cell apoptosis; apoptosis; B cell mediated immunity; brain development; circadian rhythm; dendrite regeneration; gene expression; immunoglobulin production; induction of apoptosis via death domain receptors; inflammatory cell apoptosis; inflammatory response; negative regulation of apoptosis; negative regulation of B cell activation; negative thymic T cell selection; neuron apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of MAPKKK cascade; positive regulation of protein homooligomerization; protein homooligomerization; regulation of apoptosis; regulation of caspase activity; regulation of cell proliferation; regulation of gene expression; regulation of lymphocyte differentiation; regulation of myeloid cell differentiation; renal system process; response to glucocorticoid stimulus; response to lipopolysaccharide; response to toxin; spleen development; T cell homeostasis