Western Blot (WB)
(Anti-VPS53 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:80 kDa Observed band size:95 kDa)
Rabbit anti-Human VPS53 Polyclonal Antibody | anti-VPS53 antibody
Anti-VPS53 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
VPS53; Polyclonal Antibody; Anti-VPS53 Antibody; Rabbit Polyclonal; VPS53 Antibody; Rabbit PAb; Antigen Affinity Purified; vacuolar protein sorting 53 homolog (S. cerevisiae); Anti-HCCS1 Antibody; Anti-hVps53L Antibody; Anti-PCH2E Antibody; Anti-pp13624 Antibody; anti-VPS53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human VPS53
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-VPS53 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
IP: 0.5-2uL/mg of lysate
Immunogen
E Coli-derived Human VPS53 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human VPS53 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-VPS53 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:80 kDa Observed band size:95 kDa)
Western Blot (WB)
(Anti-VPS53 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:80 kDa Observed band size:95 kDa)
Immunoprecipitation (IP)
(VPS53 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-VPS53 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS53 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa Observed band size :95 kDa)
Immunoprecipitation (IP)
(VPS53 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-VPS53 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS53 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa Observed band size :95 kDa)
Related Product Information for anti-VPS53 antibody
VPS53 (VPS53 Subunit Of GARP Complex) is a Protein Coding gene. The VPS53 gene, located on 17p13.3, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, and zebrafish, etc. VPS53 belongs to the VPS53 family. 4 alternatively spliced human isoforms have been reported. This gene encodes a protein with sequence similarity to the yeast Vps53p protein. VPS53 acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). VPS53 is widely expressed in the thyroid, brain, and other tissues. Diseases associated with VPS53 include Pontocerebellar Hypoplasia, Type 2E, and Cerebral Atrophy.
References
Xie XH, et al. (2019) Hccs1 inhibits the stemness of human pancreatic cancer stem-like cells. Eur Rev Med Pharmacol Sci 23 (5): 2125-2131.Tian MM, et al. (2017) Hepatocellular carcinoma suppressor 1 promoter hypermethylation in serum. A diagnostic and prognostic study in hepatitis b. Clin Res Hepatol Gastroenterol 41 (2): 171-180.Hausman-Kedem M, et al. (2019) Vps53 gene is associated with a new phenotype of complicated hereditary spastic paraparesis. Neurogenetics 20 (4): 187-195.
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Product Notes
The VPS53 (Catalog #AAA8108997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VPS53 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the VPS53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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