Western Blot (WB)
(Anti-PSMA4 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PSMA4 Polyclonal Antibody | anti-PSMA4 antibody
Anti-PSMA4 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PSMA4; Polyclonal Antibody; Anti-PSMA4 Antibody; Rabbit Polyclonal; PSMA4 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) subunit; alpha type; 4; Anti-HC9 Antibody; Anti-HsT17706 Antibody; Anti-PSC9 Antibody; anti-PSMA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMA4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMA4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PSMA4 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PSMA4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PSMA4 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PSMA4 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa (We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(PSMA4 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PSMA4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMA4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size :34 kDa)
Immunoprecipitation (IP)
(PSMA4 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 4 uL anti-PSMA4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMA4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size :34 kDa)
Related Product Information for anti-PSMA4 antibody
PSMA4 (Proteasome 20S Subunit Alpha 4) is a Protein Coding gene. The PSMA4 gene, located on 15q25.1, is conserved in chimpanzee, Rhesus monkey, cow, mouse, and rat, etc. This gene encodes a core alpha subunit of the 20S proteasome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. PSMA4 is widely expressed in the appendix, lymph node, and other tissues. Diseases associated with PSMA4 include Cystic Fibrosis and Angelman Syndrome. Genome-wide association studies have linked the chromosome 15q25.1 locus to lung cancer susceptibility and implicated proteasome subunit alpha type-4 (PSMA4) as a candidate gene. The related pathways of PSMA4 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation.
References
Wang T, et al. (2015) Association of psma4 polymorphisms with lung cancer susceptibility and response to cisplatin-based chemotherapy in a chinese han population. Clin Transl Oncol 17 (7): 564-569.Takabe W, et al. (2000) Gene expression induced by bo-653, probucol and bhq in human endothelial cells. J Atheroscler Thromb 7 (4): 223-230.Shabek N, et al. (2009) Ubiquitin degradation with its substrate, or as a monomer in a ubiquitination-independent mode, provides clues to proteasome regulation. Proc Natl Acad Sci U S A 106 (29): 11907-11912.
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Product Notes
The PSMA4 (Catalog #AAA8108080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMA4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PSMA4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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