Rabbit PSMA4 Polyclonal Antibody | anti-PSMA4 antibody

PSMA4 Rabbit pAb

Cat. Num. AAA4760351

• Gene Names: PSMA4; HC9; PSC9; HsT17706
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: PSMA4; Polyclonal Antibody; PSMA4 Rabbit pAb; proteasome subunit alpha 4; HC9; PSC9; HsT17706; anti-PSMA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.9mg/ml (varies by lot)

• Applicable Applications for anti-PSMA4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:40-1:200
• Immunogen: Fusion protein of human PSMA4
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of PSMA4 in NIH/3T3 cell, Mouse spleen tissue, K562 cell, Hela cell, 231 cell lysates in order using PSMA4 Rabbit pAb at dilution 1/400)

Immunohistochemistry (IHC)

(Immunohistochemistry of PSMA4 in paraffin-embedded Human liver cancer tissue using PSMA4 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm and Nucleus)

Related Product Information for anti-PSMA4 antibody

This gene encodes a core alpha subunit of the 20S proteosome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. Proteasomes cleave peptides in an ATP- and ubiquitin-dependent manner.

NCBI and Uniprot Product Information

• NCBI GI #: 130861
• NCBI GeneID: 5685
• UniProt Accession #: P25789; Q53XP2; Q567Q5; Q8TBD1; D3DW86
• Molecular Weight: 261
• NCBI Official Full Name: Proteasome subunit alpha type-4
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 4
• NCBI Official Symbol: PSMA4
• NCBI Official Synonym Symbols: HC9; PSC9; HsT17706
• NCBI Protein Information: proteasome subunit alpha type-4; macropain subunit C9; proteasome subunit L; proteasome subunit HC9; proteasome component C9; multicatalytic endopeptidase complex subunit C9
• UniProt Protein Name: Proteasome subunit alpha type-4
• Protein Family: Proteasome
• UniProt Gene Name: PSMA4
• UniProt Synonym Gene Names: HC9; PSC9
• UniProt Entry Name: PSA4_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA4: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle