Western Blot (WB)
(Anti-NT5C1A rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:44 kDa)
Rabbit NT5C1A Polyclonal Antibody | anti-NT5C1A antibody
Anti-NT5C1A Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NT5C1A; Polyclonal Antibody; Anti-NT5C1A Antibody; Rabbit Polyclonal; NT5C1A Antibody; Rabbit PAb; Antigen Affinity Purified; 5'-nucleotidase; cytosolic IA; Anti-CN-I Antibody; Anti-CN-IA Antibody; Anti-CN1 Antibody; Anti-CN1A Antibody; Anti-CNI Antibody; anti-NT5C1A antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NT5C1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NT5C1A antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human NT5C1A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NT5C1A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-NT5C1A rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:44 kDa)
Western Blot (WB)
(Anti-NT5C1A rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:44 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human NT5C1A in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NT5C1A in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NT5C1A antibody
Sporadic inclusion body myositis (sIBM) is an inflammatory myopathy (IIM) without a specific biomarker until autoantibodies to the cytosolic 5'-nucleotidase 1A (NT5c1A/Mup44) were reported. Prior investigations demonstrated that autoantibodies recognizing cytosolic 5'-nucleotidase 1A (NT5C1A) are found in 33-76% of patients with inclusion body myositis (IBM) but are observed only rarely in patients with polymyositis (PM). Thus, anti-NT5C1A may help distinguish IBM from PM. Cytosolic 5'-nucleotidase 1A (NT5C1A) dephosphorylates non-cyclic nucleoside monophosphates to produce nucleosides and inorganic phosphates. Seropositivity to the NT5c1A antibody is associated with greater motor and functional disability in sIBM. The study also suggests more prominent bulbar, facial, and respiratory involvement in individuals positive for NT5c1A antibodies.
References
Patzak MS, et al. (2019) Cytosolic 5'-nucleotidase 1a is overexpressed in pancreatic cancer and mediates gemcitabine resistance by reducing intracellular gemcitabine metabolites. EBioMedicine 40 394-405.Lloyd TE, et al. (2016) Cytosolic 5'-nucleotidase 1a as a target of circulating autoantibodies in autoimmune diseases. Arthritis Care Res (Hoboken) 68 (1): 66-71.Amlani A, et al. (2019) Anti-nt5c1a autoantibodies as biomarkers in inclusion body myositis. Front Immunol 10 745.
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Product Notes
The NT5C1A (Catalog #AAA8107994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NT5C1A Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's NT5C1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the NT5C1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5C1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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