Rabbit anti-Human F2RL3 Polyclonal Antibody | anti-F2RL3 antibody

Anti-F2RL3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101401

• Gene Names: F2RL3; PAR4
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: F2RL3; Polyclonal Antibody; Anti-F2RL3 Antibody; Rabbit Polyclonal; F2RL3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-PAR4 Antibody; anti-F2RL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human F2RL3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-F2RL3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human F2RL3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human F2RL3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-F2RL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:44 kDa)

Related Product Information for anti-F2RL3 antibody

F2R Like Thrombin/Trypsin Receptor 3 (F2RL3), also known as PAR-4, is a member of the proteinase-activated receptor subfamily of which other three members are PAR1, PAR2 and PAR3. PAR4 can be activated by trypsin, cathepsin G, the activated factor X of the coagulation cascade, and trypsin IV. Irrespective of its role in thrombin-induced platelet aggregation, PAR4 activation is believed to be involved in inflammatory lesions, as show by investigations that have unmasked the effects of PAR4 on neutrophil recruitment, the regulation of edema, and plasma extravasation. PAR4 expression has been demonstrated in various cell types including endothelial cells, neutrophils and sensory neurons. F2RL3 methylation is associated with lung cancer and cardiovascular disease in human patients.

References

Leger AJ, et al. (2006) Protease-activated receptors in cardiovascular diseases. Circulation 114 (10): 1070-1077.Bao Y, et al. (2015) New insights into protease-activated receptor 4 signaling pathways in the pathogenesis of inflammation and neuropathic pain: A literature review. Channels (Austin) 9 (1): 5-13.

NCBI and Uniprot Product Information

• NCBI GI #: 89353783
• NCBI GeneID: 9002
• NCBI Accession #: NP_003941.2
• NCBI GenBank Nucleotide #: NM_003950.2
• UniProt Accession #: Q96RI0; O76067; Q6DK42
• Molecular Weight: 41,133 Da
• NCBI Official Full Name: proteinase-activated receptor 4
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor-like 3
• NCBI Official Symbol: F2RL3
• NCBI Official Synonym Symbols: PAR4
• NCBI Protein Information: proteinase-activated receptor 4; PAR-4; thrombin receptor-like 3; protease-activated receptor-4
• UniProt Protein Name: Proteinase-activated receptor 4
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2RL3
• UniProt Synonym Gene Names: PAR4; PAR-4
• UniProt Entry Name: PAR4_HUMAN

NCBI Description

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008]

Uniprot Description

PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: thrombin receptor activity

Biological Process: platelet activation; platelet dense granule organization and biogenesis; response to wounding; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; signal transduction