Immunoprecipitation (IP)
(VTI1B was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-VTI1B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-VTI1B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)
Rabbit anti-Human VTI1B Polyclonal Antibody | anti-VTI1B antibody
Anti-VTI1B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
VTI1B; Polyclonal Antibody; Anti-VTI1B Antibody; Rabbit Polyclonal; VTI1B Antibody; Rabbit PAb; Antigen Affinity Purified; vesicle transport through interaction with t-SNAREs 1B; Anti-v-SNARE Antibody; Anti-VTI1 Antibody; Anti-VTI1-LIKE Antibody; Anti-vti1-rp1 Antibody; Anti-VTI1L Antibody; Anti-VTI2 Antibody; anti-VTI1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human VTI1B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-VTI1B antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-4uL/mg of lysate
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human VTI1B
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human VTI1B (rh VTI1B; NP_006361.1; Ala 2-Leu 208). VTI1B specific IgG was purified by Human VTI1B affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(VTI1B was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-VTI1B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-VTI1B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)
Immunoprecipitation (IP)
(VTI1B was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-VTI1B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-VTI1B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)
Western Blot (WB)
(Anti-VTI1B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-VTI1B rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-VTI1B antibody
Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. VTI1A and VTI1B are the only members of their SNARE subclass and the yeast homolog Vti1p is essential for cell survival. VTI1B has been reported to be involved in the fusion between GcAVs and lysosomes, which suggests that VTI1B regulates multiple steps of the GcAV formation process.
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Product Notes
The VTI1B (Catalog #AAA8107509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VTI1B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VTI1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the VTI1B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VTI1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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