FGFR-2(IIIb)/Fc Chimera Active Protein | FGFR-2 active protein

Recombinant Human Soluble FGFR-2(IIIb)Fc Chimera

Cat. Num. AAA692287

• Gene Names: FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
• Purity: > 90% by SDS-PAGE & silver stain
• Synonyms: FGFR-2(IIIb)/Fc Chimera; Recombinant Human Soluble FGFR-2(IIIb)Fc Chimera; Fibroblast growth factor receptor 2; BFR-1; FGFR2IIIb; KGFR; FGFR-2 active protein

• Host: Insect cells
• Purity/Purification: > 90% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: RPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEADAGEYICKVSNYIGQANQSAWLTVLPKQQAPGREKEITASPDYLEDPRRASIEGRGDPEEPKSCDKTHTCPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
• Sequence Length: 603

• Buffer: PBS
• Stabilizer: None
• Reconstitution: The lyophilized sFGFR-2(IIIb)/Fc is soluble in water and most aqueous buffers and should be reconstituted to a concentration not lower than 50?g/ml.
• Biological Activity: (1) The activity of sFGFR-2(IIIb)/Fc was determined by its ability to inhibit the FGF10-induced proliferation of 4MBr-5 cells. (2) Measured by its binding ability in a functional ELISA. Recombinant human soluble FGFR-2(IIIb)/Fc Chimera bind to immobilized recombinant human FGF-10 (MBS692030).
• Preparation and Storage: Lyophilized samples are stable for greater than six months at –20 degree C to –70 degree C. Reconstituted sFGFR-2(IIIb)/Fc should be stored in working aliquots at -20 degree C.

SDS-PAGE

(SDS-PAGE analysis of recombinant human soluble FGFR-2(IIIb)/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)

Testing Data

(Functional ELISA with recombinant human sFGFR-2(IIIb)/Fc Chimera. Recombinant human FGF-10 was coated at 2ug/ml and sFGFR2(IIIb)/Fc and sFGFR2(IIIc)/Fc were added [1ug/ml each]. FGF-10 binds only to FGFR2(IIIb) and not to FGFR2(IIIc).)

Testing Data

(Inhibition of FGF10-induced proliferation of 4MBr-5 cells by recombinant human soluble FGFR-2(IIIb)/Fc. sFGFR-2(IIIc)/Fc which do not bind FGF10 show no effect.)

Related Product Information for FGFR-2 active protein

Background: Recombinant human soluble FGFR-2 (IIIb) was fused via a Xa cleavage site with the Fc part of human IgG1. Human recombinant soluble FGFR-2 (IIIb) is a disulfide-linked homodimeric protein. In the reduced form the glycosylated subunits of sFGFR-2 (IIIb)/human Fc chimera display a molecular mass of about 90 kDa. Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGFR-1 to -4 are known. Multiple forms of FGFR-1 to -3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the ß isoform. Only the alpha isoform has been identified for FGFR-3 and FGFR-4. Additional splicing events for FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGFR-1. Mutations in FGFR-1 to -3 have been found in patients with birth defects involving craniosynostosis.

Product Categories/Family for FGFR-2 active protein

Recombinant Protein; Soluble Receptors; FGFR-2 (IIIb) /Fc Chimera; soluble

NCBI and Uniprot Product Information

• NCBI GI #: 221316639
• NCBI GeneID: 2263
• NCBI Accession #: NP_075259.4
• NCBI GenBank Nucleotide #: NM_000141.4
• UniProt Accession #: P21802; P18443; Q01742; Q12922; Q14300; Q14301; Q14302; Q14303; Q14304; B4DFC2; E7EVR6; E9PCR0
• Molecular Weight: ~90 kDa
• NCBI Official Full Name: fibroblast growth factor receptor 2 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 2
• NCBI Official Symbol: FGFR2
• NCBI Official Synonym Symbols: BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
• NCBI Protein Information: fibroblast growth factor receptor 2
• UniProt Protein Name: Fibroblast growth factor receptor 2
• UniProt Gene Name: FGFR2
• UniProt Synonym Gene Names: BEK; KGFR; KSAM; FGFR-2; KGFR
• UniProt Entry Name: FGFR2_HUMAN

Uniprot Description

FGFR2 iso3: a receptor tyrosine kinase of the highly-conserved FGFR family that binds fibroblast growth factor (FGF). Mutations are associated with many craniosynostotic syndromes and bone malformations. Mutations cause syndromes with defects in facial and limb development, including Crouzon syndrome, Beare-Stevenson cutis gyrata syndrome, Pfeiffer syndrome, Apert syndrome, and Jackson-Weiss syndrome. Somatic mutations seen in gastric cancer. Amplified in gastric, breast and some B cell cancers, but deleted in glioblastoma Twenty splice-variant isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor)

Cellular Component: cell cortex; cell surface; cytoplasm; excitatory synapse; extracellular matrix; integral to plasma membrane; intracellular membrane-bound organelle; nucleoplasm; nucleus; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; fibroblast growth factor binding; fibroblast growth factor receptor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: alveolus development; angiogenesis; axonogenesis; bone mineralization; branching morphogenesis of a nerve; cell fate commitment; cell-cell signaling; embryonic cranial skeleton morphogenesis; embryonic digestive tract morphogenesis; embryonic organ development; embryonic organ morphogenesis; embryonic pattern specification; epidermis morphogenesis; epithelial cell differentiation; fibroblast growth factor receptor signaling pathway; gland morphogenesis; gut development; hair follicle morphogenesis; in utero embryonic development; inner ear morphogenesis; lacrimal gland development; limb bud formation; lung development; MAPKKK cascade; mesenchymal cell differentiation; midbrain development; morphogenesis of embryonic epithelium; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; neuroblast division in the ventricular zone; odontogenesis; orbitofrontal cortex development; organ growth; organ morphogenesis; otic vesicle formation; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; positive regulation of cardiac muscle cell proliferation; positive regulation of cell cycle; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; post-embryonic development; protein amino acid autophosphorylation; pyramidal neuron development; regulation of cell fate commitment; regulation of fibroblast growth factor receptor signaling pathway; regulation of multicellular organism growth; regulation of osteoblast differentiation; regulation of osteoblast proliferation; regulation of phosphoinositide 3-kinase cascade; regulation of smooth muscle cell differentiation; regulation of smoothened signaling pathway; reproductive structure development; skeletal morphogenesis; ureteric bud development; ventricular cardiac muscle morphogenesis